Academic Journal
Molecular characterization of a carbon dioxide-dependent Escherichia coli small-colony variant isolated from blood cultures
| Title: | Molecular characterization of a carbon dioxide-dependent Escherichia coli small-colony variant isolated from blood cultures |
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| Authors: | Takehisa Matsumoto, Masayuki Hashimoto, Ching-Hao Teng, Po-Chuen Hsu, Yusuke Ota, Masaru Takamizawa, Ryosuke Kato, Tatsuya Negishi |
| Source: | International Journal of Medical Microbiology, Vol 310, Iss 5, Pp 151431- (2020) |
| Publisher Information: | Elsevier, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Microbiology LCC:Other systems of medicine |
| Subject Terms: | Small-colony variants, Carbonic anhydrase, can gene, Carbon dioxide-dependent, Escherichia coli, Microbiology, QR1-502, Other systems of medicine, RZ201-999 |
| More Details: | A carbon dioxide-dependent small-colony variant of Escherichia coli SH4888 was isolated from blood cultures of a patient with cholangitis. To date, little is known regarding the molecular mechanisms leading to formation of carbon dioxide-dependent phenotypes in clinical isolates, but abnormalities in the carbonic anhydrase are thought to cause carbon dioxide autotrophy. In this study DNA sequence analysis of the carbonic anhydrase-encoding can locus in the carbon dioxide-dependent E. coli SH4888 revealed that the isolate had a 325-bp deletion spanning from the 3′-terminal region of can to the 3′-terminal region of hpt, which encodes a hypoxanthine phosphoribosyltransferase. To confirm that the carbon dioxide-dependent SCV phenotype of E. coli SH4888 was due to the can mutation, we performed a complementation test with a plasmid carrying an intact can that restored the normal phenotype. However, E. coli SH4888 had increased virulence compared to the can-complemented E. coli SH4888 in a murine infection model. In conclusion, these data confirm that impaired carbonic anhydrase function can cause a carbon dioxide-dependent SCV phenotype in E. coli SH4888 and provides a fitness advantage in terms of infection. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1438-4221 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1438422120300412; https://doaj.org/toc/1438-4221 |
| DOI: | 10.1016/j.ijmm.2020.151431 |
| Access URL: | https://doaj.org/article/d1d6ea04484c4365ae08a1a231b0baeb |
| Accession Number: | edsdoj.1d6ea04484c4365ae08a1a231b0baeb |
| Database: | Directory of Open Access Journals |
| ISSN: | 14384221 |
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| DOI: | 10.1016/j.ijmm.2020.151431 |
| Published in: | International Journal of Medical Microbiology |
| Language: | English |