Bibliographic Details
| Title: |
The PolS-PolR Two-Component System Regulates Genes Involved in Poly-P Metabolism and Phosphate Transport in Microlunatus phosphovorus |
| Authors: |
Chuanqing Zhong, Peipei Zhang, Cheng Liu, Meng Liu, Wenbing Chen, Jiafang Fu, Xiaoyu Qi, Guangxiang Cao |
| Source: |
Frontiers in Microbiology, Vol 10 (2019) |
| Publisher Information: |
Frontiers Media S.A., 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
polyphosphate metabolism, two-component system, gene regulation, Microlunatus phosphovorus, phosphate transport, Microbiology, QR1-502 |
| More Details: |
Microlunatus phosphovorus NM-1 is a polyphosphate (poly-P)-accumulating bacterium that accumulates poly-P under aerobic conditions and degrades poly-P under anaerobic conditions. In this study, the two-component system (TCS) PolS-PolR was identified in NM-1, and the response regulator PolR was found to directly bind to the promoters of genes related to phosphate transport (MLP_RS00235, MLP_RS23035, and MLP_RS24590); poly-P catabolism (MLP_RS12905) and poly-P synthesis (MLP_RS23025). RT-qPCR assays showed that ppgk (MLP_RS12905), ppk (MLP_RS23025), pstS (MLP_RS23035), and pit (MLP_RS24590) were down-regulated during the aerobic-anaerobic shift. The sequence GTTCACnnnnnGTTCaC was identified as a recognition sequence for PolR by MEME analysis and DNase I footprinting. EMSAs and ChIP-qPCR assays indicated that PolR binds to the promoters of pit (MLP_RS00235), ppgk (MLP_RS12905), ppk (MLP_RS23025), pstS (MLP_RS23035) and pit (MLP_RS24590), and ChIP-qPCR further suggested that the binding affinity of PolR was lower under anaerobic conditions than under aerobic conditions in vivo. These findings indicate that the PolS-PolR TCS in M. phosphovorus may be involved in the regulation of poly-P metabolism in response to levels of dissolved oxygen in the environment, and our results provide insights into new approaches for understanding the mechanisms of phosphorus accumulation and release. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02127/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2019.02127 |
| Access URL: |
https://doaj.org/article/1d3d4faf515b4196ac1e887dac045892 |
| Accession Number: |
edsdoj.1d3d4faf515b4196ac1e887dac045892 |
| Database: |
Directory of Open Access Journals |
