Bibliographic Details
| Title: |
Vanillic Acid Reduces Pain-Related Behavior in Knee Osteoarthritis Rats Through the Inhibition of NLRP3 Inflammasome-Related Synovitis |
| Authors: |
Zhenyuan Ma, Zhengquan Huang, Li Zhang, Xiaochen Li, Bo Xu, Yancheng Xiao, Xiaoqing Shi, Haosheng Zhang, Taiyang Liao, Peimin Wang |
| Source: |
Frontiers in Pharmacology, Vol 11 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Therapeutics. Pharmacology |
| Subject Terms: |
vanillic acid, knee osteoarthritis, synovial inflammation, NLRP3 inflammasome, pain 3, Therapeutics. Pharmacology, RM1-950 |
| More Details: |
Objectives: Synovitis plays an important role in knee osteoarthritis (KOA) pain. The activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in fibroblast-like synoviocytes (FLSs) promotes KOA development. In this study, we aimed to investigate whether vanillic acid (VA), a monomer derived from Chinese herbal medicines, could target NLRP3 inflammasome-related synovitis to reduce pain.Methods: Rats in the KOA and KOA + VA groups were injected with monosodium iodoacetate (MIA) in the knee to induce KOA. From day 14, the KOA + VA group was given VA at 30 mg/kg every day via gastric intubation. FLSs were collected from the synovial tissues. We examined both the protein and gene expression of caspase-1, apoptosis-associated speck-like protein with a caspase recruitment domain (ASC), NLRP3, components of the NLRP3 inflammasome, and interleukin-1β (IL-1β) and IL-18 in vivo and in vitro.Results: The upregulation of caspase-1, ASC, and NLRP3 in the KOA model were reduced by VA. VA also lowered the level of IL-1β and IL-18 in the KOA model. In addition, VA relieved pain-related behavior of KOA model rats and downregulated the pain mediators CGRP, NGF, and TrkA in FLSs. Interestingly, we also observed reduced synovial fibrosis in the animal experiments.Conclusion: Our research showed that VA reduces synovitis and pain-related behaviors in a rat model of KOA, which provides the basis for further investigations into the potential therapeutic impact of VA in KOA. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1663-9812 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fphar.2020.599022/full; https://doaj.org/toc/1663-9812 |
| DOI: |
10.3389/fphar.2020.599022 |
| Access URL: |
https://doaj.org/article/1bd3d57b19834c98baa4e3bd388426b7 |
| Accession Number: |
edsdoj.1bd3d57b19834c98baa4e3bd388426b7 |
| Database: |
Directory of Open Access Journals |
