Academic Journal
Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps
| Title: | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
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| Authors: | Chi-Ming Pu, Ya-Chun Chen, Yu-Chen Chen, Tzu-Lin Lee, Yu-Sen Peng, Shun-Hua Chen, Yu-Hsiu Yen, Chung-Liang Chien, Jung-Hsien Hsieh, Yuh-Lien Chen |
| Source: | Mediators of Inflammation, Vol 2019 (2019) |
| Publisher Information: | Hindawi Limited, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Pathology |
| Subject Terms: | Pathology, RB1-214 |
| More Details: | The most common postoperative complication after reconstructive surgery is flap necrosis. Adipose-derived stem cells (ADSCs) and their secretomes are reported to mediate skin repair. This study was designed to investigate whether conditioned media from ADSCs (ADSC-CM) protects ischemia/reperfusion- (I/R-) induced injury in skin flaps by promoting cell proliferation and increasing the number of hair follicles. The mouse flap model of ischemia was ligating the long thoracic vessels for 3 h, followed by blood reperfusion. ADSC-CM was administered to the flaps, and their survival was observed on postoperative day 5. ADSC-CM treatment led to a significant increase in cell proliferation and the number of hair follicles. IL-6 levels in the lysate and CM from ADSCs were significantly higher than those from Hs68 fibroblasts. Furthermore, a strong decrease in cell proliferation and the number of hair follicles was observed after treatment with IL-6-neutralizing antibodies or si-IL-6-ADSC. In addition, ADSC transplantation increased flap repair, cell proliferation, and hair follicle number in I/R injury of IL-6-knockout mice. In conclusion, IL-6 secreted from ADSCs promotes the survival of I/R-induced flaps by increasing cell proliferation and the number of hair follicles. ADSCs represent a promising therapy for preventing skin flap necrosis following reconstructive and plastic surgery. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0962-9351 1466-1861 |
| Relation: | https://doaj.org/toc/0962-9351; https://doaj.org/toc/1466-1861 |
| DOI: | 10.1155/2019/2343867 |
| Access URL: | https://doaj.org/article/e1ad793b40884321a5d976e343d6582a |
| Accession Number: | edsdoj.1ad793b40884321a5d976e343d6582a |
| Database: | Directory of Open Access Journals |
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| ISSN: | 09629351 14661861 |
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| DOI: | 10.1155/2019/2343867 |
| Published in: | Mediators of Inflammation |
| Language: | English |