Bibliographic Details
| Title: |
Multicenter evaluation of four immunoassays for the performance of early diagnosis of COVID-19 and assessment of antibody responses of patients with pneumonia in Taiwan |
| Authors: |
Yi-Chun Lin, Yu-Lin Lee, Chien-Yu Cheng, Wen-Pin Tseng, Jhong-Lin Wu, Chien-Hao Lin, Ming-Yi Chung, Chun-Min Kang, Ya-Fan Lee, Cheng-Pin Chen, Chien-Hua Huang, Chun-Eng Liu, Shu-Hsing Cheng, Shyr-Chyr Chen, Shey-Ying Chen, Po-Ren Hsueh |
| Source: |
Journal of Microbiology, Immunology and Infection, Vol 54, Iss 5, Pp 816-829 (2021) |
| Publisher Information: |
Elsevier, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
COVID-19, Immunoassays, Antibody responses, False positive, Pneumonia, Microbiology, QR1-502 |
| More Details: |
Background/purpose: Our study goals were to evaluate the diagnostic performance of four anti-SARS-CoV-2 antibodies tests and the differences in dynamic immune responses between COVID-19 patients with and without pneumonia. Methods: We collected 184 serum samples from 70 consecutively qRT-PCR-confirmed COVID-19 patients at four participating hospitals from 23 January 2020 to 30 September 2020. COVID-19 pneumonia was defined as the presence of new pulmonary infiltration. Serum samples were grouped by the duration after symptom onset on a weekly basis for antibody testing and analysis. The four immunoassays: Beckman SARS-CoV-2 IgG/IgM (Beckman Test), Siemens (ADVIA Centaur®) SARS-CoV-2 Total (COV2T) (Siemens Test), SBC COVID-19 IgG ELISA (SBC Test) and EliA SARS-CoV-2-Sp1 IgG/IgM/IgA P2 Research (EliA Test) were used for detecting the SARS-CoV-2 specific antibodies. Results: The sensitivity of all tests reached 100% after 42 days of symptom onset. Siemens Test, the only test detecting total anti-SARS-CoV-2 antibodies, had the best performance in the early diagnosis of COVID-19 infection (day 0–7: 77%; day 8–14: 95%) compared to the other 3 serological tests. All tests showed 100% specificity except SBC Test (98%). COVID-19 patients with pneumonia had significantly higher testing signal values than patients without pneumonia (all p values |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1684-1182 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1684118221000463; https://doaj.org/toc/1684-1182 |
| DOI: |
10.1016/j.jmii.2021.02.003 |
| Access URL: |
https://doaj.org/article/1a62db68d0b84c3f9351d66c8cd4ba50 |
| Accession Number: |
edsdoj.1a62db68d0b84c3f9351d66c8cd4ba50 |
| Database: |
Directory of Open Access Journals |
