Academic Journal
Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro
| Title: | Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro |
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| Authors: | Wei Ge, Yuan-Chao Sun, Tian Qiao, Hai-Xia Liu, Tao-Ran He, Jun-Jie Wang, Chun-Lei Chen, Shun-Feng Cheng, Paul W. Dyce, Massimo De Felici, Wei Shen |
| Source: | Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-13 (2023) |
| Publisher Information: | BMC, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Medicine (General) LCC:Biochemistry |
| Subject Terms: | Skin-derived stem cells, Papillary dermal fibroblast progenitors, Single-cell transcriptomes, Spermatogonial stem cells like cells, Medicine (General), R5-920, Biochemistry, QD415-436 |
| More Details: | Abstract Background Many laboratories have described the in vitro isolation of multipotent cells with stem cell properties from the skin of various species termed skin-derived stem cells (SDSCs). However, the cellular origin of these cells and their capability to give rise, among various cell types, to male germ cells, remain largely unexplored. Methods SDSCs were isolated from newborn mice skin, and then differentiated into primordial germ cell-like cells (PGCLCs) in vitro. Single-cell RNA sequencing (scRNA-seq) was then applied to dissect the cellular origin of SDSCs using cells isolated from newborn mouse skin and SDSC colonies. Based on an optimized culture strategy, we successfully generated spermatogonial stem cell-like cells (SSCLCs) in vitro. Results Here, using scRNA-seq and analyzing the profile of 7543 single-cell transcriptomes from newborn mouse skin and SDSCs, we discovered that they mainly consist of multipotent papillary dermal fibroblast progenitors (pDFPs) residing in the dermal layer. Moreover, we found that epidermal growth factor (EGF) signaling is pivotal for the capability of these progenitors to proliferate and form large colonies in vitro. Finally, we optimized the protocol to efficiently generate PGCLCs from SDSCs. Furthermore, PGCLCs were induced into SSCLCs and these SSCLCs showed meiotic potential when cultured with testicular organoids. Conclusions Our findings here identify pDFPs as SDSCs derived from newborn skin and show for the first time that such precursors can be induced to generate cells of the male germline. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1757-6512 |
| Relation: | https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-023-03243-5 |
| Access URL: | https://doaj.org/article/1a3e7537a5d643ddb56fd0e6402517f7 |
| Accession Number: | edsdoj.1a3e7537a5d643ddb56fd0e6402517f7 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 17576512 |
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| DOI: | 10.1186/s13287-023-03243-5 |
| Published in: | Stem Cell Research & Therapy |
| Language: | English |