Propagation of human prostate tissue from induced pluripotent stem cells
| Title: | Propagation of human prostate tissue from induced pluripotent stem cells |
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| Authors: | Anastasia C. Hepburn, Emma L. Curry, Mohammad Moad, Rebecca E. Steele, Omar E. Franco, Laura Wilson, Parmveer Singh, Adriana Buskin, Susan E. Crawford, Luke Gaughan, Ian G. Mills, Simon W. Hayward, Craig N. Robson, Rakesh Heer |
| Source: | Stem Cells Translational Medicine, Vol 9, Iss 7, Pp 734-745 (2020) |
| Publisher Information: | Oxford University Press, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine (General) LCC:Cytology |
| Subject Terms: | androgen receptor, differentiation, induced pluripotent stem cells, organoids, prostate, prostate cancer, Medicine (General), R5-920, Cytology, QH573-671 |
| More Details: | Abstract Primary culture of human prostate organoids and patient‐derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complementary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2157-6580 2157-6564 |
| Relation: | https://doaj.org/toc/2157-6564; https://doaj.org/toc/2157-6580 |
| DOI: | 10.1002/sctm.19-0286 |
| Access URL: | https://doaj.org/article/1a016c78ab2e4635979543ad1a0a990f |
| Accession Number: | edsdoj.1a016c78ab2e4635979543ad1a0a990f |
| Database: | Directory of Open Access Journals |
| ISSN: | 21576580 21576564 |
|---|---|
| DOI: | 10.1002/sctm.19-0286 |
| Published in: | Stem Cells Translational Medicine |
| Language: | English |