Academic Journal
DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature
| Title: | DNA methylation of indoleamine 2,3-dioxygenase 1 (IDO1) in head and neck squamous cell carcinomas correlates with IDO1 expression, HPV status, patients’ survival, immune cell infiltrates, mutational load, and interferon γ signature |
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| Authors: | Verena Sailer, Ulrike Sailer, Emma Grace Bawden, Romina Zarbl, Constanze Wiek, Timo J. Vogt, Joern Dietrich, Sophia Loick, Ingela Grünwald, Marieta Toma, Carsten Golletz, Andreas Gerstner, Glen Kristiansen, Friedrich Bootz, Kathrin Scheckenbach, Jennifer Landsberg, Dimo Dietrich |
| Source: | EBioMedicine, Vol 48, Iss , Pp 341-352 (2019) |
| Publisher Information: | Elsevier, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Medicine LCC:Medicine (General) |
| Subject Terms: | Medicine, Medicine (General), R5-920 |
| More Details: | Background: The immune checkpoint, indoleamine 2,3-dioxygenase 1, is under investigation as target of novel immunotherapies for cancers, including head and neck squamous cell carcinomas (HNSCC). The aim of our study was to analyze DNA methylation of the encoding gene (IDO1) in HNSCC. Methods: Methylation of three CpG sites within the promoter, promoter flank, and gene body was investigated and correlated with mRNA expression, immune cell infiltration, mutational burden, human papillomavirus (HPV)-status, and overall survival in a cohort of N = 528 HNSCC patients obtained from The Cancer Genome Atlas. In addition, IDO1 immunohistochemistry and DNA methylation analysis was performed in an independent cohort of N = 138 HNSCC samples. Findings: Significant inverse correlations of IDO1 methylation and IDO1 mRNA expression were found in the promoter and promoter flank region (Spearman's ρ = −0.163 and ρ = −0.377, respectively) while a positive correlation was present in the gene body (ρ = 0.502; all P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2352-3964 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352396419306498; https://doaj.org/toc/2352-3964 |
| DOI: | 10.1016/j.ebiom.2019.09.038 |
| Access URL: | https://doaj.org/article/19f13459f3a443e282ec3bbaa5759ff0 |
| Accession Number: | edsdoj.19f13459f3a443e282ec3bbaa5759ff0 |
| Database: | Directory of Open Access Journals |
| ISSN: | 23523964 |
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| DOI: | 10.1016/j.ebiom.2019.09.038 |
| Published in: | EBioMedicine |
| Language: | English |