Bibliographic Details
| Title: |
SARS-CoV-2 Multi-Antigen Serology Assay |
| Authors: |
Ramin Mazhari, Shazia Ruybal-Pesántez, Fiona Angrisano, Nicholas Kiernan-Walker, Stephanie Hyslop, Rhea J. Longley, Caitlin Bourke, Catherine Chen, Deborah A. Williamson, Leanne J. Robinson, Ivo Mueller, Emily M. Eriksson |
| Source: |
Methods and Protocols, Vol 4, Iss 4, p 72 (2021) |
| Publisher Information: |
MDPI AG, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
SARS-CoV-2, COVID-19, Luminex, serology, Biology (General), QH301-705.5 |
| More Details: |
Serology tests are extremely useful for assessing whether a person has been infected with a pathogen. Since the onset of the COVID-19 pandemic, measurement of anti-SARS-CoV-2-specific antibodies has been considered an essential tool in identifying seropositive individuals and thereby understanding the extent of transmission in communities. The Luminex system is a bead-based technology that has the capacity to assess multiple antigens simultaneously using very low sample volumes and is ideal for high-throughput studies. We have adapted this technology to develop a COVID-19 multi-antigen serological assay. This protocol described here carefully outlines recommended steps to optimize and establish this method for COVID-19-specific antibody measurement in plasma and in saliva. However, the protocol can easily be customized and thus the assay is broadly applicable to measure antibodies to other pathogens. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2409-9279 |
| Relation: |
https://www.mdpi.com/2409-9279/4/4/72; https://doaj.org/toc/2409-9279 |
| DOI: |
10.3390/mps4040072 |
| Access URL: |
https://doaj.org/article/a191e41b93cf4745a71ed3402aa59310 |
| Accession Number: |
edsdoj.191e41b93cf4745a71ed3402aa59310 |
| Database: |
Directory of Open Access Journals |
