Academic Journal
Field evaluation of the performance of seven Antigen Rapid diagnostic tests for the diagnosis of SARs-CoV-2 virus infection in Uganda
| Title: | Field evaluation of the performance of seven Antigen Rapid diagnostic tests for the diagnosis of SARs-CoV-2 virus infection in Uganda |
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| Authors: | Josephine Bwogi, Tom Lutalo, Phionah Tushabe, Henry Bukenya, James Peter Eliku, Isaac Ssewanyana, Susan Nabadda, Christopher Nsereko, Matthew Cotten, Robert Downing, Julius Lutwama, Pontiano Kaleebu, EPI Laboratory team, UVRI -COVID 19 Technical team |
| Source: | PLoS ONE, Vol 17, Iss 5 (2022) |
| Publisher Information: | Public Library of Science (PLoS), 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Objective The objective of this study was to evaluate the performance of seven antigen rapid diagnostic tests (Ag RDTs) in a clinical setting to identify those that could be recommended for use in the diagnosis of SARS-CoV-2 infection in Uganda. Methods This was a cross-sectional prospective study. Nasopharyngeal swabs were collected consecutively from COVID-19 PCR positive and COVID-19 PCR negative participants at isolation centers and points of entry, and tested with the SARS-CoV-2 Ag RDTs. Test sensitivity and specificity were generated by comparing results against qRT-PCR results (Berlin Protocol) at a cycle threshold (Ct) cut-off of ≤39. Sensitivity was also calculated at Ct cut-offs ≤29 and ≤33. Results None of the Ag RDTs had a sensitivity of ≥80% at Ct cut-off values ≤33 and ≤39. Two kits, Panbio™ COVID-19 Ag and VivaDiag™ SARS-CoV-2 Ag had a sensitivity of ≥80% at a Ct cut-off value of ≤29. Four kits: BIOCREDIT COVID -19 Ag, COVID-19 Ag Respi-Strip, MEDsan® SARS-CoV-2 Antigen Rapid Test and Panbio™ COVID-19 Ag Rapid Test had a specificity of ≥97%. Conclusions This evaluation identified one Ag RDT, Panbio™ COVID-19 Ag with a performance at high viral load (Ct value ≤29) reaching that recommended by WHO. This kit was recommended for screening of patients with COVID -19-like symptoms presenting at health facilities. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1932-6203 |
| Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089886/?tool=EBI; https://doaj.org/toc/1932-6203 |
| Access URL: | https://doaj.org/article/ece18cd3159e4e23a313fdf1343211a5 |
| Accession Number: | edsdoj.18cd3159e4e23a313fdf1343211a5 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19326203 |
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| Published in: | PLoS ONE |
| Language: | English |