Academic Journal
Boosting effect of IL-7 in interferon gamma release assays to diagnose Mycobacterium tuberculosis infection.
| Title: | Boosting effect of IL-7 in interferon gamma release assays to diagnose Mycobacterium tuberculosis infection. |
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| Authors: | Hellen Hiza, Lukas Fenner, Jerry Hella, Davis Kuchaka, Mohamed Sasamalo, Thomas Blauenfeldt, Gibson Kibiki, Reginald A Kavishe, Francis Mhimbira, Morten Ruhwald |
| Source: | PLoS ONE, Vol 13, Iss 8, p e0202525 (2018) |
| Publisher Information: | Public Library of Science (PLoS), 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | BACKGROUND:A quarter of the world's population is estimated to be infected with Myobacterium tuberculosis (Mtb). Infection is detected by immune response to M. tuberculosis antigens using either tuberculin skin test (TST) and interferon gamma release (IGRA's), tests which have low sensitivity in immunocompromised. IL-7 is an important cytokine for T-cell function with potential to augment cytokine release in in-vitro assays. This study aimed to determine whether the addition of IL-7 in interferon-gamma release assays (IGRAs) improves its diagnostic performance of Mtb infection. METHODS:44 cases with confirmed TB and 45 household contacts without TB were recruited and 1ml of blood was stimulated in two separate IGRA's tube set: one set of standard Quantiferon TB gold tubes mitogen, TB antigen and TB Nil; one set of customized Quantiferon TB gold tubes with added IL-7. Following IFN-γ and IP-10 release was determined using ELISA. RESULTS:We found that the addition of IL-7 led to significantly higher release of IFN-γ in individuals with active TB from 4.2IU/ml (IQR 1.4-6.9IU/ml) to 5.1IU/ml (IQR 1.5-8.1IU/ml, p = 0.0057), and we found an indication of a lower release of both IFN-γ and IP-10 in participants with negative tests. CONCLUSIONS:In TB cases addition of IL-7 in IGRA tubes augments IFN-γ but not IP-10 release, and seems to lower the response in controls. Whether IL-7 boosted IGRA holds potential over standard IGRA needs to be confirmed in larger studies in high and low TB incidence countries. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC6114790?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0202525 |
| Access URL: | https://doaj.org/article/18487b37b47e466bb073d6830b2e396d |
| Accession Number: | edsdoj.18487b37b47e466bb073d6830b2e396d |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19326203 |
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| DOI: | 10.1371/journal.pone.0202525 |
| Published in: | PLoS ONE |
| Language: | English |