| Title: |
Spatial UBE2N protein expression indicates genomic instability in colorectal cancers |
| Authors: |
Timo Gemoll, Elena Miroll, Oliver Klein, Annette Lischka, Murat Eravci, Christoph Thorns, Jens K. Habermann |
| Source: |
BMC Cancer, Vol 19, Iss 1, Pp 1-8 (2019) |
| Publisher Information: |
BMC, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
Imaging mass spectrometry, Genomic instability, Aneuploidy, UBE2N, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
Abstract Background One major hallmark of colorectal cancers (CRC) is genomic instability with its contribution to tumor heterogeneity and therapy resistance. To facilitate the investigation of intra-sample phenotypes and the de novo identification of tumor sub-populations, imaging mass spectrometry (IMS) provides a powerful technique to elucidate the spatial distribution patterns of peptides and proteins in tissue sections. Methods In the present study, we analyzed an in-house compiled tissue microarray (n = 60) comprising CRCs and control tissues by IMS. After obtaining protein profiles through direct analysis of tissue sections, two validation sets were used for immunohistochemical evaluation. Results A total of 28 m/z values in the mass range 800–3500 Da distinguished euploid from aneuploid CRCs (p 0.635). After liquid chromatograph-mass spectrometry identification, UBE2N could be successfully validated by immunohistochemistry in the initial sample cohort (p = 0.0274, ROC AUC = 0.7937) and in an independent sample set of 90 clinical specimens (p = 0.0070, ROC AUC = 0.6957). Conclusions The results showed that FFPE protein expression profiling of surgically resected CRC tissue extracts by MALDI-TOF MS has potential value for improved molecular classification. Particularly, the protein expression of UBE2N was validated in an independent clinical cohort to distinguish euploid from aneuploid CRCs. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2407 |
| Relation: |
http://link.springer.com/article/10.1186/s12885-019-5856-1; https://doaj.org/toc/1471-2407 |
| DOI: |
10.1186/s12885-019-5856-1 |
| Access URL: |
https://doaj.org/article/e178032f73024c6dbda38eec43effe97 |
| Accession Number: |
edsdoj.178032f73024c6dbda38eec43effe97 |
| Database: |
Directory of Open Access Journals |
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