The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces
| Title: | The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces |
|---|---|
| Authors: | Sakiko Hiraoka, Shiho Takashima, Toshihiro Inokuchi, Asuka Nakarai, Masahiro Takahara, Keita Harada, Yasuhiro Seki, Katsunori Watanabe, Jun Kato, Hiroyuki Okada |
| Source: | Intestinal Research, Vol 17, Iss 2, Pp 202-209 (2019) |
| Publisher Information: | Korean Association for the Study of Intestinal Diseases, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Medicine LCC:Diseases of the digestive system. Gastroenterology |
| Subject Terms: | Colitis, ulcerative, Fecal calprotectin, Quantitative fecal immunochemical test, Latex agglutination turbidimetric immunoassay, Mucosal healing, Medicine, Diseases of the digestive system. Gastroenterology, RC799-869 |
| More Details: | Background/Aims Fecal calprotectin (Fcal) as well as the fecal immunochemical test (FIT) are useful biomarkers for detecting activity and mucosal healing in inflammatory bowel diseases. Here, we report the performance of simultaneous measurements of Fcal and FIT for ulcerative colitis (UC) patients using the newly-developed latex agglutination turbidimetric immunoassay (LATIA) system. Methods Fcal and hemoglobin were measured by the LATIA system in 152 UC patients who underwent colonoscopy. Fcal was also quantified with a conventional enzyme-linked immunosorbent assay (ELISA). Fecal markers were evaluated in conjunction with the mucosal status of UC, which was assessed via the Mayo endoscopic subscore (MES) classification. Results The LATIA system could quantify calprotectin and hemoglobin simultaneously with the same fecal samples within 10 minutes. The values of the Fcal-LATIA closely correlated with those of the Fcal-ELISA (Spearman rank correlation coefficient, r=0.84; P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1598-9100 2288-1956 |
| Relation: | http://www.irjournal.org/upload/pdf/ir-2018-00086.pdf; https://doaj.org/toc/1598-9100; https://doaj.org/toc/2288-1956 |
| DOI: | 10.5217/ir.2018.00086 |
| Access URL: | https://doaj.org/article/1751f450f5664afaba2f201689fdeac2 |
| Accession Number: | edsdoj.1751f450f5664afaba2f201689fdeac2 |
| Database: | Directory of Open Access Journals |
| ISSN: | 15989100 22881956 |
|---|---|
| DOI: | 10.5217/ir.2018.00086 |
| Published in: | Intestinal Research |
| Language: | English |