Bibliographic Details
| Title: |
Molecular epidemiology of Plasmodium vivax anti-folate resistance in India |
| Authors: |
Dev Vas, Joshi Hema, Prajapati Surendra K, Dua Virendra K |
| Source: |
Malaria Journal, Vol 10, Iss 1, p 102 (2011) |
| Publisher Information: |
BMC, 2011. |
| Publication Year: |
2011 |
| Collection: |
LCC:Arctic medicine. Tropical medicine
LCC:Infectious and parasitic diseases |
| Subject Terms: |
Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216 |
| More Details: |
Abstract Background Sulphadoxine and pyrimethamine are anti-folate drugs that show synergistic anti-malarial effect. Point mutations in dihydrofolate reductase (dhfr) and dihydropteorate synthatase (dhps) cause anti-folate drug resistance phenotype in human malaria parasites. This study presents pattern of point mutations in dhfr/dhps genes among Indian sub-continent. Methods Microscopically diagnosed one hundred Plasmodium vivax field isolates were collected from five widely separated geographical regions of India. Dhfr and dhps genes were PCR amplified and sequenced. Previously published mutations data were collected and analyzed using Chi square test to identify geographical cluster of mutant/wild type genotypes. Results Sequence analysis revealed single (S58R), double (S58R/S117N) and quadruple (F57L/S58R/T61M/S117T/) point mutations at dhfr and single (A383G) to double (A383G/A553G) mutations at dhps in P. vivax field isolates. In addition, three new mutations were also observed at dhfr. Both, dhfr and dhps genes revealed tandem repeat variations in field isolates. Dhps revealed very low mutation frequency (14.0%) compared to dhfr (50.70%). Comparative analysis revealed a progressive increase in frequency of quadruple mutant dhfr genotype (p < 0.001) within five years in north-eastern state (Kamrup, Assam). Frequency of dhfr genotypes revealed three distinct geographical clusters of wild (northern India), double mutant (southern India), and quadruple mutant (north-eastern and island regions of India) on the Indian sub-continent. Conclusion Study suggests that SP may be susceptible to P. vivax in India, except Andaman and north-eastern state. The distinction of geographical regions with sensitive and resistant parasite phenotypes would be highly useful for designing and administering national anti-malarial drug policy. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1475-2875 |
| Relation: |
http://www.malariajournal.com/content/10/1/102; https://doaj.org/toc/1475-2875 |
| DOI: |
10.1186/1475-2875-10-102 |
| Access URL: |
https://doaj.org/article/aaeeadc162c941658c0ce5c068015c7c |
| Accession Number: |
edsdoj.162c941658c0ce5c068015c7c |
| Database: |
Directory of Open Access Journals |
