Bibliographic Details
| Title: |
B cells and ectopic follicular structures: novel players in anti-tumor programming with prognostic power for patients with metastatic colorectal cancer. |
| Authors: |
Anastasia Meshcheryakova, Dietmar Tamandl, Erika Bajna, Judith Stift, Martina Mittlboeck, Martin Svoboda, Denise Heiden, Stefan Stremitzer, Erika Jensen-Jarolim, Thomas Grünberger, Michael Bergmann, Diana Mechtcheriakova |
| Source: |
PLoS ONE, Vol 9, Iss 6, p e99008 (2014) |
| Publisher Information: |
Public Library of Science (PLoS), 2014. |
| Publication Year: |
2014 |
| Collection: |
LCC:Medicine
LCC:Science |
| Subject Terms: |
Medicine, Science |
| More Details: |
Remarkably limited information is available about biological mechanisms that determine the disease entity of metastatic colorectal cancer in the liver (CRCLM) with no good clinical parameters to estimate prognosis. For the last few years, understanding the relationship between tumor characteristics and local immune response has gained increasing attention. Given the multifaceted roles of B-cell-driven responses, we aimed to elucidate the immunological imprint of B lymphocytes at the metastatic site, the interrelation with macrophages, and their prognostic relevance. Here we present novel algorithm allowing to assess a link between the local patient-specific immunological capacity and clinical outcome. The microscopy-based imaging platform was used for automated scanning of large-scale tissue sections and subsequent qualitative and quantitative analyses of immune cell subtypes using lineage markers and single-cell recognition strategy. Results indicate massive infiltration of CD45-positive leukocytes confined to the metastatic border. We report for the first time the accumulation of CD20-positive B lymphocytes at the tumor-liver interface comprising the major population within the large CD45-positive aggregates. Strikingly, functionally active, activation-induced cytidine deaminase (AID)-positive ectopic lymphoid structures were found to be assembled within the metastatic margin. Furthermore, the CD20-based data set revealed a strong prognostic power: patients with high CD20 content and/or ectopic follicles had significantly lower risk for disease recurrence as revealed by univariate analysis (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1932-6203 |
| Relation: |
http://europepmc.org/articles/PMC4048213?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: |
10.1371/journal.pone.0099008 |
| Access URL: |
https://doaj.org/article/15fe33fa18164c5abef55d14debcdc35 |
| Accession Number: |
edsdoj.15fe33fa18164c5abef55d14debcdc35 |
| Database: |
Directory of Open Access Journals |
