Bibliographic Details
| Title: |
A Finger-Actuated Sample-Dosing Capillary-Driven Microfluidic Device for Loop-Mediated Isothermal Amplification |
| Authors: |
Xuan Le, Jianxiong Chan, James McMahon, Jessica A. Wisniewski, Anna Coldham, Tuncay Alan, Patrick Kwan |
| Source: |
Biosensors, Vol 14, Iss 9, p 410 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Biotechnology |
| Subject Terms: |
point-of-care testing, POCT, loop-mediated isothermal, LAMP, finger-actuated microfluidic, capillary-driven microfluidic, Biotechnology, TP248.13-248.65 |
| More Details: |
Loop-mediated isothermal amplification (LAMP) has attracted significant attention for rapid and accurate point-of-care diagnostics. However, integrating sample introduction, lysis, amplification, and detection steps into an easy-to-use, disposable system has so far been challenging. This has limited the uptake of the technique in practical applications. In this study, we developed a colourimetric one-step LAMP assay that combines thermolysis and LAMP reaction, to detect the SARS-CoV-2 virus in nasopharyngeal swab samples from COVID-19-infected individuals. The limit of detection was 500 copies per reaction at 65 °C for 25 min in reaction tubes. Additionally, we developed a finger-operated capillary-driven microfluidic device with selective PVA coating. This finger-actuated microfluidic device could self-dose the required sample amount for the LAMP reaction and inhibit sample evaporation. Finally, we integrated the LAMP assay into the microfluidic device by short-term pre-storage of the LAMP master mix. Using this device, nasopharyngeal swab samples from COVID-19-infected individuals showed positive results at a reaction time of 35 min at 65 °C. This integrated device may be adapted to detect other RNA viruses of interest rapidly. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2079-6374 |
| Relation: |
https://www.mdpi.com/2079-6374/14/9/410; https://doaj.org/toc/2079-6374 |
| DOI: |
10.3390/bios14090410 |
| Access URL: |
https://doaj.org/article/15081fb0dfce4aeea1848390b547f5ff |
| Accession Number: |
edsdoj.15081fb0dfce4aeea1848390b547f5ff |
| Database: |
Directory of Open Access Journals |
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