Bibliographic Details
| Title: |
Cayratia albifolia C.L.Li exerts anti-rheumatoid arthritis effect by inhibiting macrophage activation and neutrophil extracellular traps (NETs) |
| Authors: |
Wei Wang, Zai-Qi Zhang, Yi-Chi Zhang, Yi-Qiang Wu, Zhuo Yang, Yong-Zhe Zheng, Jia-Hong Lu, Peng-Fei Tu, Ke-Wu Zeng |
| Source: |
Chinese Medicine, Vol 19, Iss 1, Pp 1-13 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Other systems of medicine |
| Subject Terms: |
Rheumatoid arthritis, Cayratia albifolia C.L.Li, Macrophage, PI3K-Akt pathway, NETosis, Other systems of medicine, RZ201-999 |
| More Details: |
Abstract Background Cayratia albifolia C.L.Li (CAC), commonly known as “Jiao-Mei-Gu” in China, has been extensively utilized by the Dong minority for several millennia to effectively alleviate symptoms associated with autoimmune diseases. CAC extract is believed to possess significant anti-inflammatory properties within the context of Dong medicine. However, an in-depth understanding of the specific pharmaceutical effects and underlying mechanisms through which CAC extract acts against rheumatoid arthritis (RA) has yet to be established. Methods Twenty-four Sprague–Dawley rats were divided into four groups, with six rats in each group. To induce the collagen-induced arthritis (CIA) model, the rats underwent a process of double immunization with collagen and adjuvant. CAC extract (100 mg/kg) was orally administered to rats. The anti-RA effects were evaluated in CIA rats by arthritis score, hind paw volume and histopathology analysis. Pull-down assay was conducted to identify the potential targets of CAC extract from RAW264.7 macrophage lysates. Moreover, mechanism studies of CAC extract were performed by immunofluorescence assays, real-time PCR and Western blot. Results CAC extract was found to obviously down-regulate hind paw volume of CIA rats, with diminished inflammation response and damage. 177 targets were identified from CAC extract by MS-based pull-down assay. Bioinformatics analysis found that these targets were mainly enriched in macrophage activation and neutrophils extracellular traps (NETs). Additionally, we reported that CAC extract owned significant anti-inflammatory activity by regulating PI3K-Akt-mTOR signal pathway, and inhibited NETosis in response to PMA. Conclusions We clarified that CAC extract significantly attenuated RA by inactivating macrophage and reducing NETosis via a multi-targets regulation. Graphical Abstract |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1749-8546 |
| Relation: |
https://doaj.org/toc/1749-8546 |
| DOI: |
10.1186/s13020-024-00910-4 |
| Access URL: |
https://doaj.org/article/a1477b7f6c6b4f1e8476560913090881 |
| Accession Number: |
edsdoj.1477b7f6c6b4f1e8476560913090881 |
| Database: |
Directory of Open Access Journals |
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