Bibliographic Details
| Title: |
Complete genetic characterization of carbapenem-resistant Acinetobacter johnsonii, co-producing NDM-1, OXA-58, and PER-1 in a patient source |
| Authors: |
Chongmei Tian, Jianqin Song, Lingzhi Ren, Delian Huang, Siwei Wang, Liping Fu, Yaping Zhao, Yongfeng Bai, Xueyu Fan, Tianhong Ma, Junjie Ying |
| Source: |
Frontiers in Cellular and Infection Microbiology, Vol 13 (2023) |
| Publisher Information: |
Frontiers Media S.A., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Acinetobacter johnsonii, carbapenem resistance, NDM-1, OXA-58, PER-1, integron, Microbiology, QR1-502 |
| More Details: |
The emergence of carbapenemase-producing Acinetobacter spp. has been widely reported and become a global threat. However, carbapenem-resistant A. johnsonii strains are relatively rare and without comprehensive genetic structure analysis, especially for isolates collected from human specimen. Here, one A. johnsonii AYTCM strain, co-producing NDM-1, OXA-58, and PER-1 enzymes, was isolated from sputum in China in 2018. Antimicrobial susceptibility testing showed that it was resistant to meropenem, imipenem, ceftazidime, ciprofloxacin, and cefoperazone/sulbactam. Whole-genome sequencing and bioinformatic analysis revealed that it possessed 11 plasmids. blaOXA-58 and blaPER-1 genes were located in the pAYTCM-1 plasmid. Especially, a complex class 1 integron consisted of a 5′ conserved segment (5′ CS) and 3′ CS, which was found to carry sul1, arr-3, qnrVC6, and blaPER-1 cassettes. Moreover, the blaNDM-1 gene was located in 41,087 conjugative plasmids and was quite stable even after 70 passages under antibiotics-free conditions. In addition, six prophage regions were identified. Tracking of closely related plasmids in the public database showed that pAYTCM-1 was similar to pXBB1-9, pOXA23_010062, pOXA58_010030, and pAcsw19-2 plasmids, which were collected from the strains of sewage in China. Concerning the pAYTCM-3 plasmids, results showed that strains were collected from different sources and their hosts were isolated from various countries, such as China, USA, Japan, Brazil, and Mexico, suggesting that a wide spread occurred all over the world. In conclusion, early surveillance is warranted to avoid the extensive spread of this high-risk clone in the healthcare setting. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2235-2988 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fcimb.2023.1227063/full; https://doaj.org/toc/2235-2988 |
| DOI: |
10.3389/fcimb.2023.1227063 |
| Access URL: |
https://doaj.org/article/1453dd8ccfc0438595f7c3444c5593e0 |
| Accession Number: |
edsdoj.1453dd8ccfc0438595f7c3444c5593e0 |
| Database: |
Directory of Open Access Journals |
