| Title: |
Integration of intermittent calcium signals in T cells revealed by temporally patterned optogenetics |
| Authors: |
Béatrice Corre, Yassine El Janati Elidrissi, Justine Duval, Mailys Quilhot, Gaëtan Lefebvre, Solène Ecomard, Fabrice Lemaître, Zacarias Garcia, Armelle Bohineust, Erica Russo, Philippe Bousso |
| Source: |
iScience, Vol 26, Iss 2, Pp 106068- (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Science |
| Subject Terms: |
Biological sciences, Biochemistry, Immunology, Immunological methods, Science |
| More Details: |
Summary: T cells become activated following one or multiple contacts with antigen-presenting cells. Calcium influx is a key signaling event elicited during these cellular interactions; however, it is unclear whether T cells recall and integrate calcium signals elicited during temporally separated contacts. To study the integration of calcium signals, we designed a programmable, multiplex illumination strategy for temporally patterned optogenetics (TEMPO). We found that a single round of calcium elevation was insufficient to promote nuclear factor of activated T cells (NFAT) activity and cytokine production in a T cell line. However, robust responses were detected after a second identical stimulation even when signals were separated by several hours. Our results suggest the existence of a biochemical memory of calcium signals in T cells that favors signal integration during temporally separated contacts and promote cytokine production. As illustrated here, TEMPO is a versatile approach for dissecting temporal integration in defined signaling pathways. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004223001451; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2023.106068 |
| Access URL: |
https://doaj.org/article/129b59cdef164681884d2a17a20e5701 |
| Accession Number: |
edsdoj.129b59cdef164681884d2a17a20e5701 |
| Database: |
Directory of Open Access Journals |
