Academic Journal
Brain type of creatine kinase induces doxorubicin resistance via TGF-β signaling in MDA-MB-231 breast cancer cells
| Title: | Brain type of creatine kinase induces doxorubicin resistance via TGF-β signaling in MDA-MB-231 breast cancer cells |
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| Authors: | Seogho Son, Seung-ah Yoo, KeeSoo Nam, Sunhwa Oh, Kyung-min Lee, Jae Youn Yi, Incheol Shin |
| Source: | Animal Cells and Systems, Vol 26, Iss 5, Pp 203-213 (2022) |
| Publisher Information: | Taylor & Francis Group, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine (General) LCC:Biology (General) |
| Subject Terms: | Apoptosis, brain type of creatine kinase (CKB), doxorubicin, MDA-MB-231, transforming growth factor-β (TGF-β), Medicine (General), R5-920, Biology (General), QH301-705.5 |
| More Details: | Brain type of creatine kinase (CKB) regulates energy homeostasis by reversibly transferring phosphate groups between phosphocreatine and ATP at sites of high energy demand. Several types of cancer cells exhibit upregulated CKB expression, but the function of CKB in cancer cells remains unclear. In this study, we investigated the function of CKB in breast cancer by overexpressing CKB in MDA-MB-231 cells. The overexpression of CKB did not affect cell growth rate, cell cycle distribution, ATP level or key mediators of aerobic glycolysis and lactate dehydrogenase isoform levels. Meanwhile, CKB overexpression did increase resistance to doxorubicin. TGF-β-induced Smad phosphorylation and Smad-dependent transcriptional activity were significantly up-regulated by CKB expression without changes in inhibitory Smad protein levels. Moreover, treatment with TGF-β considerably enhanced cell viability during doxorubicin treatment and decreased doxorubicin-induced apoptosis in CKB-expressing MDA-MB-231 cells compared to control cells. These results suggest that CKB attenuates doxorubicin-induced apoptosis and potentiates resistance to doxorubicin by enhancing TGF-β signaling in MDA-MB-231 cells. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 19768354 2151-2485 1976-8354 |
| Relation: | https://doaj.org/toc/1976-8354; https://doaj.org/toc/2151-2485 |
| DOI: | 10.1080/19768354.2022.2107070 |
| Access URL: | https://doaj.org/article/12819d0484a149a6ac75fd6c9a9818cd |
| Accession Number: | edsdoj.12819d0484a149a6ac75fd6c9a9818cd |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19768354 21512485 |
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| DOI: | 10.1080/19768354.2022.2107070 |
| Published in: | Animal Cells and Systems |
| Language: | English |