Academic Journal
Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.)
| Title: | Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.) |
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| Authors: | Deyuan Meng, Aamana Batool, Yazhou Xuan, Ruiqing Pan, Na Zhang, Wei Zhang, Liya Zhi, Xiaoli Ren, Wenqing Li, Jijie Li, Yanxiao Niu, Shuzhi Zheng, Jun Ji, Xiaoli Shi, Lei Wang, Hongqing Ling, Chunhua Zhao, Fa Cui, Xigang Liu, Junming Li, Liqiang Song |
| Source: | Crop Journal, Vol 11, Iss 5, Pp 1491-1500 (2023) |
| Publisher Information: | KeAi Communications Co., Ltd., 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Agriculture LCC:Agriculture (General) |
| Subject Terms: | Wheat, Thousand-kernel weight, Fine mapping, Candidate gene, Agriculture, Agriculture (General), S1-972 |
| More Details: | Thousand-kernel weight (TKW) is a measure of grain weight, a target of wheat breeding. The object of this study was to fine-map a stable quantitative trait loci (QTL) for TKW and identify its candidate gene in a recombinant inbred line (RIL) population derived from the cross of Kenong 9204 (KN9204) and Jing 411 (J411). On a high-density genetic linkage map, 24, 26 and 25 QTL were associated with TKW, kernel length (KL), and kernel width (KW), respectively. A major and stable QTL, QTkw-2D, was mapped to an 8.3 cM interval on chromosome arm 2DL. By saturation of polymorphic markers in its target region, QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line (F6:7). This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines (NILs). NILsKN9204 had higher fresh and dry weights than NILsJ411 at various grain-filling stages. The TKW and KW of NILsKN9204 were much higher than those of NILsJ411 in field trials. By comparison of both DNA sequence and expression between KN9204 and J411, TraesCS2D02G460300.1 (TraesKN2D01HG49350) was assigned as a candidate gene for QTkw-2D. This was confirmed by RNA sequencing (RNA-seq) of QTkw-2D NILs. These results provide the basis of map-based cloning of QTkw-2D, and DNA markers linked to the candidate gene may be used in marker-assisted selection. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2214-5141 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2214514123000375; https://doaj.org/toc/2214-5141 |
| DOI: | 10.1016/j.cj.2023.03.007 |
| Access URL: | https://doaj.org/article/d1174bfa38d94898ad65f93d1e31db13 |
| Accession Number: | edsdoj.1174bfa38d94898ad65f93d1e31db13 |
| Database: | Directory of Open Access Journals |
| ISSN: | 22145141 |
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| DOI: | 10.1016/j.cj.2023.03.007 |
| Published in: | Crop Journal |
| Language: | English |