Metformin promotes susceptibility to experimental Leishmania braziliensis infection
| Title: | Metformin promotes susceptibility to experimental Leishmania braziliensis infection |
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| Authors: | Filipe Rocha Lima, Lais de Melo Ferreira, Tainá Alves Malta, Icaro Bonyek-Silva, Reinan Lima Santos, Natália Machado Tavares, Edgar Marcelino de Carvalho Filho, Sérgio Arruda |
| Source: | Memorias do Instituto Oswaldo Cruz, Vol 115 (2020) |
| Publisher Information: | Fundação Oswaldo Cruz (FIOCRUZ), 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| Subject Terms: | metformin, cutaneous leishmaniasis, immunomodulation, susceptibility, infection, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| More Details: | BACKGROUND Metformin (MET) is a hypoglycemic drug used for the treatment of diabetes, despite interference in host immunity against microorganisms. Cutaneous infection caused by pathogens such as Leishmania braziliensis (Lb), the agent responsible for cutaneous leishmaniasis (CL) in Brazil, represents an interesting model in which to evaluate the effects associated with MET. OBJECTIVE To evaluate the modulatory effect of MET in Lb infection. MATERIAL AND METHODS Experimental study of Lb infection and MET treatment in BALB/c mice and Raw 264.7 macrophages. FINDINGS MET treatment interfered with lesion kinetics, increased parasite load and reduced macrophage proliferation. Low concentrations of MET in Lb culture allow for the maintenance of stationary parasite growth phase. Lb-infected cells treated with MET exhibited increased parasite load. While both MET and Lb infection alone promoted the production of intracellular reactive oxygen species (ROS), reduced levels of ROS were seen in MET-treated Lb-infected macrophages. MAIN CONCLUSION Experimental treatment with MET interfered with the kinetics of cutaneous ulceration, increased Lb parasite load, altered ROS production and modulated cellular proliferation. Our experimental results indicate that MET interfere with the evolution of CL. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1678-8060 0074-0276 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100353&tlng=en; http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e200272.pdf; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/0074-02760200272 |
| Access URL: | https://doaj.org/article/c1018031bab74e9eaafee9a4017c08b4 |
| Accession Number: | edsdoj.1018031bab74e9eaafee9a4017c08b4 |
| Database: | Directory of Open Access Journals |
| ISSN: | 16788060 00740276 |
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| DOI: | 10.1590/0074-02760200272 |
| Published in: | Memorias do Instituto Oswaldo Cruz |
| Language: | English |