Fusion of Normoxic- and Hypoxic-Preconditioned Myoblasts Leads to Increased Hypertrophy

Bibliographic Details
Title: Fusion of Normoxic- and Hypoxic-Preconditioned Myoblasts Leads to Increased Hypertrophy
Authors: Tamara Pircher, Henning Wackerhage, Elif Akova, Wolfgang Böcker, Attila Aszodi, Maximilian M. Saller
Source: Cells, Vol 11, Iss 6, p 1059 (2022)
Publisher Information: MDPI AG, 2022.
Publication Year: 2022
Collection: LCC:Cytology
Subject Terms: C2C12, myoblasts, myotube, fusion, myogenic differentiation, hypoxia, Cytology, QH573-671
More Details: Injuries, high altitude, and endurance exercise lead to hypoxic conditions in skeletal muscle and sometimes to hypoxia-induced local tissue damage. Thus, regenerative myoblasts/satellite cells are exposed to different levels and durations of partial oxygen pressure depending on the spatial distance from the blood vessels. To date, it is unclear how hypoxia affects myoblasts proliferation, differentiation, and particularly fusion with normoxic myoblasts. To study this, we investigated how 21% and 2% oxygen affects C2C12 myoblast morphology, proliferation, and myogenic differentiation and evaluated the fusion of normoxic- or hypoxic-preconditioned C2C12 cells in 21% or 2% oxygen in vitro. Out data show that the long-term hypoxic culture condition does not affect the proliferation of C2C12 cells but leads to rounder cells and reduced myotube formation when compared with myoblasts exposed to normoxia. However, when normoxic- and hypoxic-preconditioned myoblasts were differentiated together, the resultant myotubes were significantly larger than the control myotubes. Whole transcriptome sequencing analysis revealed several novel candidate genes that are differentially regulated during the differentiation under normoxia and hypoxia in mixed culture conditions and may thus be involved in the increase in myotube size. Taken together, oxygen-dependent adaption and interaction of myoblasts may represent a novel approach for the development of innovative therapeutic targets.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2073-4409
Relation: https://www.mdpi.com/2073-4409/11/6/1059; https://doaj.org/toc/2073-4409
DOI: 10.3390/cells11061059
Access URL: https://doaj.org/article/0f30fd9d6ae047d79e1e9e578ba8d7c4
Accession Number: edsdoj.0f30fd9d6ae047d79e1e9e578ba8d7c4
Database: Directory of Open Access Journals
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More Details
ISSN:20734409
DOI:10.3390/cells11061059
Published in:Cells
Language:English