Bibliographic Details
| Title: |
Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells |
| Authors: |
Yi-Ning Chen, Hsiao-Chin Hsu, Sheng-Wei Wang, Hao-Chiang Lien, Hsin-Ti Lu, Sheng-Kai Peng |
| Source: |
Pathogens, Vol 8, Iss 4, p 259 (2019) |
| Publisher Information: |
MDPI AG, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
scotophilus bat coronavirus-512, severe acute respiratory syndrome-coronavirus, pseudovirus, cell receptor, Medicine |
| More Details: |
Bats are natural reservoirs of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome CoV (MERS-CoV). Scotophilus bat CoV-512 demonstrates potential for cross-species transmission because its viral RNA and specific antibodies have been detected in three bat species of Taiwan. Understanding the cell tropism of Scotophilus bat CoV-512 is the first step for studying the mechanism of cross-species transmission. In this study, a lentivirus-based pseudovirus was produced using the spike (S) protein of Scotophilus bat CoV-512 or SARS-CoV as a surface protein to test the interaction between coronaviral S protein and its cell receptor on 11 different cells. Susceptible cells expressed red fluorescence protein (RFP) after the entry of RFP-bound green fluorescence protein (GFP)-fused S protein of Scotophilus bat CoV-512 (RFP-Sco-S-eGFP) or RFP-SARS-S pseudovirus, and firefly luciferase (FLuc) activity expressed by cells infected with FLuc-Sco-S-eGFP or FLuc-SARS-S pseudovirus was quantified. Scotophilus bat CoV-512 pseudovirus had significantly higher entry efficiencies in Madin Darby dog kidney epithelial cells (MDCK), black flying fox brain cells (Pabr), and rat small intestine epithelial cells (IEC-6). SARS-CoV pseudovirus had significantly higher entry efficiencies in human embryonic kidney epithelial cells (HEK-293T), pig kidney epithelial cells (PK15), and MDCK cells. These findings demonstrated that Scotophilus bat CoV-512 had a broad host range for cross-species transmission like SARS-CoV. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2076-0817 |
| Relation: |
https://www.mdpi.com/2076-0817/8/4/259; https://doaj.org/toc/2076-0817 |
| DOI: |
10.3390/pathogens8040259 |
| Access URL: |
https://doaj.org/article/0e9abf9814fd4449baf14eeb914e7a16 |
| Accession Number: |
edsdoj.0e9abf9814fd4449baf14eeb914e7a16 |
| Database: |
Directory of Open Access Journals |
