Bibliographic Details
| Title: |
Establishment and Validation of Patient-Derived Non-Small Cell Lung Cancer Organoids as In Vitro Lung Cancer Models |
| Authors: |
Raphael S. Werner, Jae-Hwi Jang, Markus Rechsteiner, Michaela B. Kirschner, Isabelle Opitz |
| Source: |
Organoids, Vol 3, Iss 4, Pp 281-294 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Cytology |
| Subject Terms: |
non-small cell lung cancer, cancer organoids, cancer model, stem cells, intratumoral heterogeneity, Cytology, QH573-671 |
| More Details: |
Background: Recent advances in the personalized treatment of non-small cell lung cancer (NSCLC) require representative in vitro model systems that reflect tumor heterogeneity and maintain the characteristic genetic aberrations. We therefore aimed to establish patient-derived NSCLC organoids that offer a reliable platform for further investigations. Methods: NSCLC organoids were cultured between May 2020 and February 2022 from surgically resected NSCLC tissue specimens. After histological and immunohistochemical validation, genetic validation was performed by targeted next-generation sequencing of tissue and organoid specimens using the Oncomine Focus Assay (ThermoFisher Scientific). Results: From 37 resected NSCLC samples, 18 primary organoid cultures were successfully established and expanded during early passages. Upon histomorphological validation, organoids showed complementary characteristics when compared to the resected parental tumor, including adenocarcinoma, squamous cell carcinoma, mucoepidermoid carcinoma, and lung carcinoid differentiation. Among nine parental tumors, traceable genetic alterations were detected, and three corresponding organoids lines retained this mutational profile, including a KRAS p.Gly12Val mutation, KRAS p.Gly12Cys mutation, and RET-fusion. Conclusions: The establishment of primary NSCLC organoids from surgically resected tissue is feasible. Histological, immunohistochemical, and genetic validation is essential to identify representative NSCLC organoids that maintain the characteristics of the parental tumor. Overall, low establishment rates remain a challenge for broad clinical applications. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2674-1172 |
| Relation: |
https://www.mdpi.com/2674-1172/3/4/17; https://doaj.org/toc/2674-1172 |
| DOI: |
10.3390/organoids3040017 |
| Access URL: |
https://doaj.org/article/0e982bea40f2412e8e62e1898be15e96 |
| Accession Number: |
edsdoj.0e982bea40f2412e8e62e1898be15e96 |
| Database: |
Directory of Open Access Journals |
