Bibliographic Details
| Title: |
Visual detection of West Nile virus using reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip |
| Authors: |
Zengguo eCao, Hualei eWang, Lina eWang, Ling eLi, Hongli eJin, Na eFeng, Changping eXu, Jianzhong eWang, Qian eLi, Yongkun eZhao, Tiecheng eWang, Yuwei eGao, Yiyu eLu, Songtao eYang, Xianzhu eXia |
| Source: |
Frontiers in Microbiology, Vol 7 (2016) |
| Publisher Information: |
Frontiers Media S.A., 2016. |
| Publication Year: |
2016 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
West Nile virus, visual detection, Reverse transcription loop-mediated isothermal amplification, visualization strip, RT-LAMP-VF, Microbiology, QR1-502 |
| More Details: |
West Nile virus (WNV) causes a severe zoonosis, which can lead to a large number of casualties and considerable economic losses. A rapid and accurate identification methodfor WNV for use in field laboratories is urgently needed. Here, a method utilizing reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip (RT-LAMP-VF) was developed to detect the envelope (E) gene of WNV. The RT-LAMP-VF assay could detect 102 copies/μl ofan WNV RNA standard using a 40 min amplification reaction followed by a 2 min incubationof the amplification product on the visualization strip, and no cross-reaction with other closely related members of theFlavivirus genus was observed. The assay was further evaluated using cells and mouse brain tissues infected with a recombinant rabies virus expressing the E protein of WNV.The assay produced sensitivities of 101.5TCID50/ml and 101.33 TCID50/ml for detection of the recombinant virus in the cells and brain tissues, respectively. Overall, the RT-LAMP-VF assay developed in this study is rapid, simple and effective, and it is therefore suitable for clinical application in the field. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-302X |
| Relation: |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00554/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2016.00554 |
| Access URL: |
https://doaj.org/article/0cb9b0d9546a4fd6aa02157a4987b746 |
| Accession Number: |
edsdoj.0cb9b0d9546a4fd6aa02157a4987b746 |
| Database: |
Directory of Open Access Journals |
