Academic Journal
Molecular identification and functional characterization of a cyanogenic glucosyltransferase from flax (Linum unsitatissimum).
| Title: | Molecular identification and functional characterization of a cyanogenic glucosyltransferase from flax (Linum unsitatissimum). |
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| Authors: | Michael Kazachkov, Qiang Li, Wenyun Shen, Liping Wang, Peng Gao, Daoquan Xiang, Raju Datla, Jitao Zou |
| Source: | PLoS ONE, Vol 15, Iss 2, p e0227840 (2020) |
| Publisher Information: | Public Library of Science (PLoS), 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Flax seed has become consumers' choice for not only polyunsaturated alpha-linolenic fatty acid but also nutraceuticals such as lignans and soluble fiber. There is, however, a major drawback of flax as a source of functional food since the seeds contain significant level of cyanogenic glucosides. The final step of cyanogenic glucoside biosynthesis is mediated by UDP-glucose dependent glucosyltransferase. To date, no flax cyanogenic glucosyl transferase genes have been reported with verified biochemical functionality. Here we present a study on the identification and enzymatic characterization of a first flax cyanogenic glucosyltransferase, LuCGT1. We show that LuCGT1 was highly active towards both aliphatic and aromatic substrates. The LuCGT1 gene is expressed in leaf tissues as well as in developing seeds, and its expression level was drastically reduced in flax mutant lines low in cyanogenic glucosides. Identification of LuCGT1 provides a molecular handle for developing gene specific markers for targeted breeding of low cyanogenic glucosides in flax. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0227840 |
| Access URL: | https://doaj.org/article/0c605c929bf04b279ba0407e7866a607 |
| Accession Number: | edsdoj.0c605c929bf04b279ba0407e7866a607 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19326203 |
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| DOI: | 10.1371/journal.pone.0227840 |
| Published in: | PLoS ONE |
| Language: | English |