Bibliographic Details
| Title: |
MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB |
| Authors: |
Xiaoxiao Gao, Xiangbin You, Guowei Wang, Mengtian Liu, Longlong Ye, Yufeng Meng, Gan Luo, Dequan Xu, Min Liu |
| Source: |
Veterinary Research, Vol 55, Iss 1, Pp 1-10 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Veterinary medicine |
| Subject Terms: |
miR-320, CCAAT enhancer binding protein beta, PRRSV, ORF6, pig, Veterinary medicine, SF600-1100 |
| More Details: |
Abstract Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease caused by Porcine reproductive and respiratory syndrome virus (PRRSV), results in huge economic losses to the world pig industry. MiRNAs have been reported to be involved in regulation of viral infection. In our study, miR-320 was one of 21 common differentially expressed miRNAs of Meishan, Pietrain, and Landrace pig breeds at 9-h post-infection (hpi). Bioinformatics and experiments found that PRRSV replication was inhibited by miR-320 through directly targeting PRRSV ORF6. In addition, the expression of CCAAT enhancer binding protein beta (CEBPB) was also inhibited by miR-320 by targeting the 3สน UTR of CEBPB, which significantly promotes PRRSV replication. Intramuscular injection of pEGFP-N1-miR-320 verified that miR-320 significantly inhibited the replication of PRRSV and alleviated the symptoms caused by PRRSV in piglets. Taken together, miR-320 have significant roles in the infection and may be promising therapeutic target for PRRS. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1297-9716 |
| Relation: |
https://doaj.org/toc/1297-9716 |
| DOI: |
10.1186/s13567-024-01309-7 |
| Access URL: |
https://doaj.org/article/d0c36a6965e8445f981fcb9ff44eac57 |
| Accession Number: |
edsdoj.0c36a6965e8445f981fcb9ff44eac57 |
| Database: |
Directory of Open Access Journals |
