Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense
| Title: | Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense |
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| Authors: | Jin-Li Ding, Jia Hou, Ming-Guang Feng, Sheng-Hua Ying |
| Source: | Virulence, Vol 11, Iss 1, Pp 1352-1365 (2020) |
| Publisher Information: | Taylor & Francis Group, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Infectious and parasitic diseases |
| Subject Terms: | insect immunity, hemocyte response, encapsulation, cell-wall protein, entomopathogenic fungus, beauveria bassiana, virulence, Infectious and parasitic diseases, RC109-216 |
| More Details: | Entomopathogenic fungi naturally infect insect hosts in environment. Fungal invasion and host immune defense are still in the progress of co-evolution. In this study, entomopathogenic fungus Beauveria bassiana and lepidopteran insect Galleria mellonella were used to investigate host cellular immunity and fungal strategy to evade host defense. First of all, genome-wide expression revealed the transcriptomic responses of hemocytes to insect mycopathogen, which dynamically varied during infection process. Enrichment analysis indicated that differentially expressed genes were primarily involved in metabolism, cellular process and immune system. Notably, cellular response involved a series of hydrolytic enzyme and antimicrobial peptide genes which were sorted together in clustering analysis. In B. bassiana, a cell-wall protein gene (BbCwp) contributes to fungal development in host hemocoel and virulence. RT-qPCR analyses indicated that infection by ΔBbCwp mutant strain caused the up-regulated expression of a series of immunity-related genes, including β-1, 3-glucan recognition protein, hydrolytic enzyme and antimicrobial peptide genes. Disruption of BbCwp resulted in a significant change in conidial lectin-binding feature and the enhanced encapsulation by the host hemocytes. After being treated with hydrolytic enzymes, ΔBbCwp mutant displayed a significantly enhanced sensitivity to osmotic and oxidative stresses. In conclusion, fungal invasion initiates comprehensive physiological responses in the host hemocytes. For mycopathogen, cell-wall protein plays an important role in fungal evasion of immunity defense and colonization in host. Our studies provide an initial framework for exploring more mechanistic details about the fungus–host interaction. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2150-5594 2150-5608 21505594 |
| Relation: | https://doaj.org/toc/2150-5594; https://doaj.org/toc/2150-5608 |
| DOI: | 10.1080/21505594.2020.1827886 |
| Access URL: | https://doaj.org/article/0b6b44bf4bca42ca950665a29d2b3786 |
| Accession Number: | edsdoj.0b6b44bf4bca42ca950665a29d2b3786 |
| Database: | Directory of Open Access Journals |
| ISSN: | 21505594 21505608 |
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| DOI: | 10.1080/21505594.2020.1827886 |
| Published in: | Virulence |
| Language: | English |