An RNA Motif That Enables Optozyme Control and Light‐Dependent Gene Expression in Bacteria and Mammalian Cells
| Title: | An RNA Motif That Enables Optozyme Control and Light‐Dependent Gene Expression in Bacteria and Mammalian Cells |
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| Authors: | Georg Pietruschka, Américo T. Ranzani, Anna Weber, Tejal Patwari, Sebastian Pilsl, Christian Renzl, David M. Otte, Daniel Pyka, Andreas Möglich, Günter Mayer |
| Source: | Advanced Science, Vol 11, Iss 12, Pp n/a-n/a (2024) |
| Publisher Information: | Wiley, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Science |
| Subject Terms: | aptamers, LOV‐domains, optogenetics, ribozymes, SELEX, synthetic biology, Science |
| More Details: | Abstract The regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells. Optoribogenetics extends this principle by molecular RNA devices acting on the RNA level whose functions are controlled by the photoinduced interaction of a light‐oxygen‐voltage photoreceptor with cognate RNA aptamers. Here light‐responsive ribozymes, denoted optozymes, which undergo light‐dependent self‐cleavage and thereby control gene expression are described. This approach transcends existing aptamer‐ribozyme chimera strategies that predominantly rely on aptamers binding to small molecules. The optozyme method thus stands to enable the graded, non‐invasive, and spatiotemporally resolved control of gene expression. Optozymes are found efficient in bacteria and mammalian cells and usher in hitherto inaccessible optoribogenetic modalities with broad applicability in synthetic and systems biology. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2198-3844 20230451 |
| Relation: | https://doaj.org/toc/2198-3844 |
| DOI: | 10.1002/advs.202304519 |
| Access URL: | https://doaj.org/article/0b5e94232b5f4f0da9d2e77feec018fa |
| Accession Number: | edsdoj.0b5e94232b5f4f0da9d2e77feec018fa |
| Database: | Directory of Open Access Journals |
| ISSN: | 21983844 20230451 |
|---|---|
| DOI: | 10.1002/advs.202304519 |
| Published in: | Advanced Science |
| Language: | English |