Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center
| Title: | Prevalence of laboratory markers of human respiratory viruses in monkeys of Adler primate center |
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| Authors: | L. I. Korzaya, D. I. Dogadov, A. M. Goncharenko, A. A. Karlsen, K. K. Kyuregyan, M. I. Mikhailov |
| Source: | Вопросы вирусологии, Vol 66, Iss 6, Pp 425-433 (2022) |
| Publisher Information: | Central Research Institute for Epidemiology, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Microbiology |
| Subject Terms: | human respiratory viruses – influenza a, influenza b, parainfluenza types 1, 2, 3, 4, respiratory syncytial (rs) virus, adenovirus, measles virus, antibodies (igg, igm), enzyme-linked immunosorbent assay (elisa), monkeys, Microbiology, QR1-502 |
| More Details: | Introduction. The relevance of studying the circulation of human respiratory viruses among laboratory primates is associated with the need to test vaccines and antiviral drugs against these infections on monkeys. The aim of this work was to study the prevalence of serological and molecular markers of human respiratory viral infections in laboratory primates born at the Adler Primate Center and in imported monkeys. Material and methods. Blood serum samples (n = 1971) and lung autopsy material (n = 26) were obtained from different monkey species. These samples were tested for the presence of serological markers of measles, parainfluenza (PI) types 1, 2, 3, influenza A and B, respiratory syncytial (RS) and adenovirus infections using enzyme immunoassay (ELISA). Detection of RS virus, metapneumovirus, PI virus types 1–4, rhinovirus, coronavirus, and adenoviruses B, C, E and bocavirus nucleic acids in this material was performed by reverse transcription polymerase chain reaction (RT-PCR). Results and discussion. The overall prevalence of antibodies (Abs) among all monkeys was low and amounted 11.3% (95% CI: 9.2–13.7%, n = 811) for measles virus, 8.9% (95% CI: 6.2–12.2%, n = 381) for PI type 3 virus, 2.5% (95% CI: 0.8–5.6%, n = 204) for PI type 1 virus, and 7.7% (95% CI: 3.8–13.7%, n = 130) for adenoviruses. When testing 26 autopsy lung samples from monkeys of different species that died from pneumonia, 2 samples from Anubis baboons (Papio аnubis) were positive for of parainfluenza virus type 3 RNA. Conclusion. Our data suggest the importance of the strict adherence to the terms of quarantine and mandatory testing of monkey sera for the presence of IgM antibodies to the measles virus that indicate the recent infection. The role of PI virus type 3 in the pathology of the respiratory tract in Anubis baboons has been established. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Russian |
| ISSN: | 0507-4088 2411-2097 |
| Relation: | https://virusjour.crie.ru/jour/article/viewFile/576/354; https://doaj.org/toc/0507-4088; https://doaj.org/toc/2411-2097 |
| DOI: | 10.36233/0507-4088-77 |
| Access URL: | https://doaj.org/article/0a991515c8df441aae478f8382e270a9 |
| Accession Number: | edsdoj.0a991515c8df441aae478f8382e270a9 |
| Database: | Directory of Open Access Journals |
| ISSN: | 05074088 24112097 |
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| DOI: | 10.36233/0507-4088-77 |
| Published in: | Вопросы вирусологии |
| Language: | English Russian |