Bibliographic Details
| Title: |
Cefazolin and imipenem enhance AmpC expression and resistance in NagZ-dependent manner in Enterobacter cloacae complex |
| Authors: |
Xianggui Yang, Zhenguo Wang, Mingquan Liu, Xuejing Yu, Yuanxiu Zhong, Fuying Wang, Ying Xu |
| Source: |
BMC Microbiology, Vol 22, Iss 1, Pp 1-11 (2022) |
| Publisher Information: |
BMC, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Cefazolin, Imipenem, AmpC, NagZ, Enterobacter cloacae complex, Microbiology, QR1-502 |
| More Details: |
Abstract Background Enterobacter cloacae complex (ECC) is a common opportunistic pathogen and is responsible for causing various infections in humans. Owing to its inducible chromosomal AmpC β-lactamase (AmpC), ECC is inherently resistant to the 1st- and 2nd- generation cephalosporins. However, whether β-lactams antibiotics enhance ECC resistance remains unclear. Results In this study, we found that subinhibitory concentrations (SICs) of cefazolin (CFZ) and imipenem (IMP) can advance the expression of AmpC and enhance its resistance towards β-lactams through NagZ in Enterobacter cloacae (EC). Further, AmpC manifested a substantial upregulation in EC in response to SICs of CFZ and IMP. In nagZ knockout EC (ΔnagZ), the resistance to β-lactam antibiotics was rather weakened and the effect of CFZ and IMP on AmpC induction was completely abrogated. NagZ ectopic expression can rescue the induction effects of CFZ and IMP on AmpC and increase ΔnagZ resistance. More importantly, CFZ and IMP have the potential to induce the expression of AmpR's target genes in a NagZ-dependent manner. Conclusions Our findings suggest that NagZ is a critical determinant for CFZ and IMP to promote AmpC expression and resistance and that CFZ and IMP should be used with caution since they may aggravate ECC resistance. At the same time, this study further improves our understanding of resistance mechanisms in ECC. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1471-2180 |
| Relation: |
https://doaj.org/toc/1471-2180 |
| DOI: |
10.1186/s12866-022-02707-7 |
| Access URL: |
https://doaj.org/article/d0a10c29dfa14460a3f70f4590528fe0 |
| Accession Number: |
edsdoj.0a10c29dfa14460a3f70f4590528fe0 |
| Database: |
Directory of Open Access Journals |
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