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Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage

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Title: Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage
Authors: Guan-gui Chen, Min Mao, Li-zi Qiu, Qi-ming Liu
Source: Neural Regeneration Research, Vol 10, Iss 3, Pp 425-431 (2015)
Publisher Information: Wolters Kluwer Medknow Publications, 2015.
Publication Year: 2015
Collection: LCC:Neurology. Diseases of the nervous system
Subject Terms: tamoxifen, Src kinase, PP2, trauma, regeneration, neuroprotection, auranofin, dextromethorphan, rosiglitazone, Alzheimer′s disease, neuroinflammation, neurodegeneration, microglia, astrocytes, nerve regeneration, spinal cord, electroacupuncture therapy, neural stem cells, notch signaling pathway, inflammation, survival curve, proliferation, differentiation, real-time quantitative PCR, western blot assay, neural regeneration, superparamagnetic iron oxide, magnetic guidance, bone marrow mesenchymal stem cells, spinal cord injury, cell transplantation, magnetic resonance image, lumbar puncture, spinal cord transection, average combined score, magnetic resonance imaging, diffusion tensor imaging, fractional anisotropy, apparent diffusion coefficient, fiber tractography, peripheral nerve injury, sciatic nerve, hypothermia, blood-nerve barrier, Evans blue tracer, neural degeneration, polyethyleneimine-polyethylene glycol, spiral ganglion cells, X-linked inhibitor of apoptosis protein, gene therapy, nanocarrier, cisplatin, ototoxicity, cochlea, Neurology. Diseases of the nervous system, RC346-429
More Details: Polyethyleneimine-polyethylene glycol (PEI-PEG), a novel nanocarrier, has been used for transfection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein (XIAP) in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Auditory brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1673-5374
Relation: http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=3;spage=425;epage=431;aulast=Chen; https://doaj.org/toc/1673-5374
DOI: 10.4103/1673-5374.153691
Access URL: https://doaj.org/article/aed090bcdcae4040b185840218a5d7db
Accession Number: edsdoj.090bcdcae4040b185840218a5d7db
Database: Directory of Open Access Journals
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  Data: Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage
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  Data: Polyethyleneimine-polyethylene glycol (PEI-PEG), a novel nanocarrier, has been used for transfection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein (XIAP) in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Auditory brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.
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        Value: 10.4103/1673-5374.153691
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 425
    Subjects:
      – SubjectFull: tamoxifen
        Type: general
      – SubjectFull: Src kinase
        Type: general
      – SubjectFull: PP2
        Type: general
      – SubjectFull: trauma
        Type: general
      – SubjectFull: regeneration
        Type: general
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        Type: general
      – SubjectFull: auranofin
        Type: general
      – SubjectFull: dextromethorphan
        Type: general
      – SubjectFull: rosiglitazone
        Type: general
      – SubjectFull: Alzheimer′s disease
        Type: general
      – SubjectFull: neuroinflammation
        Type: general
      – SubjectFull: neurodegeneration
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      – SubjectFull: microglia
        Type: general
      – SubjectFull: astrocytes
        Type: general
      – SubjectFull: nerve regeneration
        Type: general
      – SubjectFull: spinal cord
        Type: general
      – SubjectFull: electroacupuncture therapy
        Type: general
      – SubjectFull: neural stem cells
        Type: general
      – SubjectFull: notch signaling pathway
        Type: general
      – SubjectFull: inflammation
        Type: general
      – SubjectFull: survival curve
        Type: general
      – SubjectFull: proliferation
        Type: general
      – SubjectFull: differentiation
        Type: general
      – SubjectFull: real-time quantitative PCR
        Type: general
      – SubjectFull: western blot assay
        Type: general
      – SubjectFull: neural regeneration
        Type: general
      – SubjectFull: superparamagnetic iron oxide
        Type: general
      – SubjectFull: magnetic guidance
        Type: general
      – SubjectFull: bone marrow mesenchymal stem cells
        Type: general
      – SubjectFull: spinal cord injury
        Type: general
      – SubjectFull: cell transplantation
        Type: general
      – SubjectFull: magnetic resonance image
        Type: general
      – SubjectFull: lumbar puncture
        Type: general
      – SubjectFull: spinal cord transection
        Type: general
      – SubjectFull: average combined score
        Type: general
      – SubjectFull: magnetic resonance imaging
        Type: general
      – SubjectFull: diffusion tensor imaging
        Type: general
      – SubjectFull: fractional anisotropy
        Type: general
      – SubjectFull: apparent diffusion coefficient
        Type: general
      – SubjectFull: fiber tractography
        Type: general
      – SubjectFull: peripheral nerve injury
        Type: general
      – SubjectFull: sciatic nerve
        Type: general
      – SubjectFull: hypothermia
        Type: general
      – SubjectFull: blood-nerve barrier
        Type: general
      – SubjectFull: Evans blue tracer
        Type: general
      – SubjectFull: neural degeneration
        Type: general
      – SubjectFull: polyethyleneimine-polyethylene glycol
        Type: general
      – SubjectFull: spiral ganglion cells
        Type: general
      – SubjectFull: X-linked inhibitor of apoptosis protein
        Type: general
      – SubjectFull: gene therapy
        Type: general
      – SubjectFull: nanocarrier
        Type: general
      – SubjectFull: cisplatin
        Type: general
      – SubjectFull: ototoxicity
        Type: general
      – SubjectFull: cochlea
        Type: general
      – SubjectFull: Neurology. Diseases of the nervous system
        Type: general
      – SubjectFull: RC346-429
        Type: general
    Titles:
      – TitleFull: Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage
        Type: main
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              M: 01
              Type: published
              Y: 2015
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              Value: 10
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            – TitleFull: Neural Regeneration Research
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