Bibliographic Details
| Title: |
Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage |
| Authors: |
Guan-gui Chen, Min Mao, Li-zi Qiu, Qi-ming Liu |
| Source: |
Neural Regeneration Research, Vol 10, Iss 3, Pp 425-431 (2015) |
| Publisher Information: |
Wolters Kluwer Medknow Publications, 2015. |
| Publication Year: |
2015 |
| Collection: |
LCC:Neurology. Diseases of the nervous system |
| Subject Terms: |
tamoxifen, Src kinase, PP2, trauma, regeneration, neuroprotection, auranofin, dextromethorphan, rosiglitazone, Alzheimer′s disease, neuroinflammation, neurodegeneration, microglia, astrocytes, nerve regeneration, spinal cord, electroacupuncture therapy, neural stem cells, notch signaling pathway, inflammation, survival curve, proliferation, differentiation, real-time quantitative PCR, western blot assay, neural regeneration, superparamagnetic iron oxide, magnetic guidance, bone marrow mesenchymal stem cells, spinal cord injury, cell transplantation, magnetic resonance image, lumbar puncture, spinal cord transection, average combined score, magnetic resonance imaging, diffusion tensor imaging, fractional anisotropy, apparent diffusion coefficient, fiber tractography, peripheral nerve injury, sciatic nerve, hypothermia, blood-nerve barrier, Evans blue tracer, neural degeneration, polyethyleneimine-polyethylene glycol, spiral ganglion cells, X-linked inhibitor of apoptosis protein, gene therapy, nanocarrier, cisplatin, ototoxicity, cochlea, Neurology. Diseases of the nervous system, RC346-429 |
| More Details: |
Polyethyleneimine-polyethylene glycol (PEI-PEG), a novel nanocarrier, has been used for transfection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein (XIAP) in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Auditory brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1673-5374 |
| Relation: |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2015;volume=10;issue=3;spage=425;epage=431;aulast=Chen; https://doaj.org/toc/1673-5374 |
| DOI: |
10.4103/1673-5374.153691 |
| Access URL: |
https://doaj.org/article/aed090bcdcae4040b185840218a5d7db |
| Accession Number: |
edsdoj.090bcdcae4040b185840218a5d7db |
| Database: |
Directory of Open Access Journals |
