| Title: |
Hazardous Effects of Sucralose and Its Disinfection Byproducts Identified From an E. coli Whole-Cell Array Analysis |
| Authors: |
Yuanxin Zhai, Dan Bai, Heyun Yang, Xiaoliang Li, Daiwen Zhu, Xin Cao, Hao Ma, Xiaolin Li, Xing Zheng |
| Source: |
Frontiers in Environmental Science, Vol 9 (2021) |
| Publisher Information: |
Frontiers Media S.A., 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Environmental sciences |
| Subject Terms: |
sucralose, disinfection byproducts, E. coli whole-cell microarray assay, toxicogenomics, toxicity mechanism, Environmental sciences, GE1-350 |
| More Details: |
In this study, an E. coli whole-cell microarray assay was used to evaluate the impact of sucralose (SUC) on 110 selected genes under different exposure concentrations. Furthermore, toxicity caused by SUC under five disinfection processes was explored. Our results revealed detailed transcriptional information for gaining insights into the toxicity mechanism. SUC at lower concentrations tends to induce more protein response, whereas greater DNA damage occurs at higher concentrations. In addition, SUC could induce changes in the expression of various genes, with the DNA damage exhibiting an obvious concentration dependence. As the exposure concentration of SUC increases, stress is transformed from a single-type level to the entire system, which could enhance cellular ability to resist damage and survive. Exposure–recovery tests show that long-term exposure (24 h) to SUC causes irreversible damage, and at SUC concentrations of 103–104 mg/L, short-term exposure (2 h) exerts the same effect. Furthermore, SUC toxicity is enhanced on disinfection, with ultraviolet light causing particularly serious DNA damage. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2296-665X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fenvs.2021.724685/full; https://doaj.org/toc/2296-665X |
| DOI: |
10.3389/fenvs.2021.724685 |
| Access URL: |
https://doaj.org/article/c08213de2d7a436ca5e73e5b50950911 |
| Accession Number: |
edsdoj.08213de2d7a436ca5e73e5b50950911 |
| Database: |
Directory of Open Access Journals |
