Bibliographic Details
| Title: |
Inhibitory Effects of Metformin for Pancreatic Neuroendocrine Neoplasms: Experimental Study on Mitochondrial Function |
| Authors: |
Shogo Maruzen, Seiichi Munesue, Mitsuyoshi Okazaki, Satoshi Takada, Shinichi Nakanuma, Isamu Makino, Linxiang Gong, Susumu Kohno, Chiaki Takahashi, Hidehiro Tajima, Yasuhiko Yamamoto, Shintaro Yagi |
| Source: |
Onco, Vol 4, Iss 2, Pp 77-86 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
panNEN, progression-free survival, metformin, AMPK activation, suppressed mitochondrial respiration, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
Although pancreatic neuroendocrine neoplasms (panNENs) are much less common and have a better prognosis than exocrine pancreatic cancers, their recurrence rate is not low, even in Grade 1 (World Health Organization classification) panNEN. Recently, there have been several reports that the progression-free survival in patients with unresectable panNEN could be improved by an antidiabetic drug, metformin, with the co-treatment of everolimus or a somatostatin analog. In this study, we aimed to evaluate the effects of metformin on cell metabolism and viability using the panNEN cell line, QGP-1, and RIN-m in culture. We observed an inhibitory effect of metformin on QGP-1 cell proliferation in a dose-dependent manner. Metformin was found to decrease the oxygen consumption rate in QGP-1 and RIN-m cells after metformin 48 h treatment and immediately after exposure. Cell proliferation was suppressed after metformin treatment. Phosphorylated adenosine monophosphate-activated protein kinase (AMPK) expression was increased, and cyclin D1 expression was decreased in RIN-m cells 24 h after metformin treatment by Western blotting in a dose-dependent manner. In conclusion, suppressive mitochondrial respiration and AMPK activation by metformin are, thus, suggested to inhibit panNEN cell viability and cell survival. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2673-7523 |
| Relation: |
https://www.mdpi.com/2673-7523/4/2/7; https://doaj.org/toc/2673-7523 |
| DOI: |
10.3390/onco4020007 |
| Access URL: |
https://doaj.org/article/07262afaca4441f986249be1464e7ebd |
| Accession Number: |
edsdoj.07262afaca4441f986249be1464e7ebd |
| Database: |
Directory of Open Access Journals |
