Bibliographic Details
| Title: |
The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility |
| Authors: |
Sara Stigliani, Adriana Amaro, Francesco Reggiani, Elena Maccarini, Claudia Massarotti, Matteo Lambertini, Paola Anserini, Paola Scaruffi |
| Source: |
Basic and Clinical Andrology, Vol 34, Iss 1, Pp 1-7 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Medicine (General) |
| Subject Terms: |
Human sperm, Cryopreservation, Storage time, Microarray, Transcriptome, Fertility preservation, Medicine (General), R5-920 |
| More Details: |
Abstract Background Cryopreservation of human spermatozoa is a widely used technique in the assisted reproduction technology laboratory for the storage of gametes for later use, for the fertility preservation and for sperm donation programs. Cryopreservation can cause damage to membrane, cytoskeletal, acrosome and increased oxidative stress, sperm DNA damage and transcriptome changes. To assess the impact of storage time on the transcriptome of frozen human spermatozoa, semen samples were collected from 24 normospermic donors of whom 13 had cryostored semen for a short-time (1 week) and 11 had cryostored semen for a long-time (median 9 years). Results RNA was extracted from each frozen-thawed sperm sample, randomized in pools, and analyzed by microarrays. Five transcripts were in higher abundance in the long-time respect to the short-time storage group. Functional annotation enrichment disclosed that that the length of cryostorage has no effect on critical pathways involved in sperm physiology and function. Conclusions The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2051-4190 |
| Relation: |
https://doaj.org/toc/2051-4190 |
| DOI: |
10.1186/s12610-024-00231-4 |
| Access URL: |
https://doaj.org/article/a070fe51d6054922bc31272776fe3c9d |
| Accession Number: |
edsdoj.070fe51d6054922bc31272776fe3c9d |
| Database: |
Directory of Open Access Journals |
