Identification and Characterization of P0 Protein as a Vaccine Candidate Against Babesia divergens, Blood Parasite of Veterinary and Zoonotic Importance

Bibliographic Details
Title: Identification and Characterization of P0 Protein as a Vaccine Candidate Against Babesia divergens, Blood Parasite of Veterinary and Zoonotic Importance
Authors: Shimaa Abd El-Salam El-Sayed, Mohamed Abdo Rizk, Haitham Eldoumani, Shimaa Sobhy Sorour, Mohamad Alaa Terkawi, Mahmoud AbouLaila, Ikuo Igarashi, Mohamed Z. Sayed-Ahmed
Source: Frontiers in Veterinary Science, Vol 8 (2022)
Publisher Information: Frontiers Media S.A., 2022.
Publication Year: 2022
Collection: LCC:Veterinary medicine
Subject Terms: Babesia divergens, P0 protein, vaccine, zoonotic infection, diagnosis, Veterinary medicine, SF600-1100
More Details: The molecular identification and antigenic characterization of P0 protein in Babesia divergens, a blood parasite of veterinary and zoonotic importance, were carried out in this study for use in developing subunit vaccines against B. divergens infection. Recombinant protein encoding P0 (BdP0) was developed in Escherichia coli, and its antiserum was generated in mice for further molecular characterization. Anti-rBdP0 serum had a specific interaction with the corresponding legitimate B. divergens protein, as confirmed by Western blotting and indirect fluorescent antibody tests. ELISA was used to assess the immunogenicity of BdP0 in a group of 68 bovine field samples, and significant immunological reactivity was found in 19 and 20 positive samples of rBdp0 and B. divergens lysate, respectively. The in vitro growth of B. divergens cultures treated with anti-rBdP0 serum was significantly inhibited (p < 0.05). Furthermore, after 6 h of incubation with 2 mg/ml anti-rBdP0 serum, the ability of pre-incubated free merozoites to invade bovine erythrocytes was reduced by 59.88%. The obtained data suggest the possible use of rBdP0 as diagnostic antigen and may serve as a vaccine candidate against babesiosis caused by B. divergens either in animal or human.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2297-1769
Relation: https://www.frontiersin.org/articles/10.3389/fvets.2021.795906/full; https://doaj.org/toc/2297-1769
DOI: 10.3389/fvets.2021.795906
Access URL: https://doaj.org/article/06d41afe7f0e4356b1378cf5ac7de45b
Accession Number: edsdoj.06d41afe7f0e4356b1378cf5ac7de45b
Database: Directory of Open Access Journals
More Details
ISSN:22971769
DOI:10.3389/fvets.2021.795906
Published in:Frontiers in Veterinary Science
Language:English