| Title: |
Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9 |
| Authors: |
Anna Wagner, Jana Seiler, Martin Beye |
| Source: |
G3: Genes, Genomes, Genetics, Vol 12, Iss 6 (2022) |
| Publisher Information: |
Oxford University Press, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Genetics |
| Subject Terms: |
Genetics, QH426-470 |
| More Details: |
AbstractFunctional genetic studies in honeybees have been limited to transposon mediated transformation and site directed mutagenesis tools. However, site- and sequence-specific manipulations that insert DNA fragments or replace sequences at specific target sites are lacking. Such tools would enable the tagging of proteins, the expression of reporters and site-specific amino acid changes, which are all gold standard manipulations for physiological, organismal, and genetic studies. However, such manipulations must be very efficient in honeybees since screening and crossing procedures are laborious due to their social organization. Here, we report an accurate and remarkably efficient site-specific integration of DNA-sequences into the honeybee genome using clustered regularly interspaced short palindromic repeat/clustered regularly interspaced short palindromic repeat-associated protein 9-mediated homology-directed repair. We employed early embryonic injections and selected a highly efficient sgRNA in order to insert 294 and 729 bp long DNA sequences into a specific locus at the dsx |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2160-1836 |
| Relation: |
https://doaj.org/toc/2160-1836 |
| DOI: |
10.1093/g3journal/jkac098 |
| Access URL: |
https://doaj.org/article/06bb97b1014b48168457eba181cb6647 |
| Accession Number: |
edsdoj.06bb97b1014b48168457eba181cb6647 |
| Database: |
Directory of Open Access Journals |
