Academic Journal
View in EDS

Comparison of Six Antifungal Susceptibilities of 11 Candida Species Using the VITEK2 AST–YS08 Card and Broth Microdilution Method

Bibliographic Details
Title: Comparison of Six Antifungal Susceptibilities of 11 Candida Species Using the VITEK2 AST–YS08 Card and Broth Microdilution Method
Authors: Hyeyoung Lee, Seong Hyouk Choi, Junsang Oh, Jehyun Koo, Hyun Ji Lee, Sung–Il Cho, Jeong Hwan Shin, Hae Kyung Lee, Soo–Young Kim, Chae Hoon Lee, Young Ree Kim, Yong–Hak Sohn, Woo Jin Kim, Sook Won Ryu, Gi–Ho Sung, Jayoung Kim
Source: Microbiology Spectrum, Vol 10, Iss 2 (2022)
Publisher Information: American Society for Microbiology, 2022.
Publication Year: 2022
Collection: LCC:Microbiology
Subject Terms: Candida, antifungal susceptibility, Vitek 2 AST–YS08, broth microdilution, epidemiological cutoff value, Microbiology, QR1-502
More Details: ABSTRACT We used a Vitek 2 AST–YS08 (YS08) system and the broth microdilution method (BMD) adopted by the Clinical and Laboratory Standards Institute (CLSI) to compare the susceptibility of 184 isolates of 11 Candida species to fluconazole, voriconazole, micafungin, caspofungin, amphotericin B, and flucytosine. In Candida albicans, the categorical agreement (CA) was 79.2%, 91.7%, 95.8%, and 95.8% for fluconazole, voriconazole, micafungin, and caspofungin, respectively. About 12.5% and 4.2% of very major errors were detected for fluconazole and voriconazole, respectively. C. glabrata showed excellent essential agreements (EAs) (>90%) for azoles but different MIC distributions for fluconazole and caspofungin. The CA between BMD fluconazole MICs and YS08 voriconazole MICs by the method-specific clinical breakpoint (CBP) was 90% in C. glabrata. Over 80% of C. glabrata and C. krusei isolates identified as micafungin–susceptible were labeled intermediate or resistant to caspofungin in YS08. In C. parapsilosis, 5.3% of very major errors and 10.5% of minor errors were found, whereas 33.3% of minor errors were observed in C. tropicalis for fluconazole. For C. tropicalis, 13 (61.9%) non-wild type (WT) isolates of fluconazole and 7 (33.3%) non-WTs of voriconazole were classified in YS08 as WT. For C. auris, the EAs were 93.3%, 100%, 82.2%, 97.8%, and 97.8% for fluconazole, voriconazole, micafungin, caspofungin, and amphotericin B, respectively. YS08 showed comparable results to the BMD. However, considering the lower YS08 fluconazole MIC results compared with BMD in Candida species and YS08 caspofungin results in C. glabrata and C. krusei, improvements are needed. IMPORTANCE The new Vitek 2 AST–YS08 (YS08) card has been updated to reflect the recently revised Clinical and Laboratory Standards Institute (CLSI) guideline. In this study, antifungal drug susceptibility tests were performed using the YS08 card and compared with the CLSI broth microdilution (BMD) method. In conclusion, YS08 showed similar results to BMD, including with C. auris. However, about 12.5% and 4.2% of major errors were detected for fluconazole and voriconazole, respectively, in C. albicans. More than 80% of C. glabrata and C. krusei isolates identified as susceptible to micafungin were labeled moderate or resistant to caspofungin in YS08. The categorical agreement between BMD fluconazole MICs and YS08 voriconazole MICs was 90% by the method-specific CBP of voriconazole, 80% by the current epidemiological cutoff value (ECV) (0.25 μg/mL) of voriconazole, and 85% by the previous ECV (0.5 μg/mL) of voriconazole. Further improvements in YS08 for the detection of fluconazole and echinocandin resistance are thus needed.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2165-0497
Relation: https://doaj.org/toc/2165-0497
DOI: 10.1128/spectrum.01253-21
Access URL: https://doaj.org/article/063889040e6f4076bf1a09f49bc5b6c8
Accession Number: edsdoj.063889040e6f4076bf1a09f49bc5b6c8
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://resolver.ebsco.com/c/xy5jbn/result?sid=EBSCO:edsdoj&genre=article&issn=21650497&ISBN=&volume=10&issue=2&date=20220401&spage=&pages=&title=Microbiology Spectrum&atitle=Comparison%20of%20Six%20Antifungal%20Susceptibilities%20of%2011%20Candida%20Species%20Using%20the%20VITEK2%20AST%E2%80%93YS08%20Card%20and%20Broth%20Microdilution%20Method&aulast=Hyeyoung%20Lee&id=DOI:10.1128/spectrum.01253-21
    Name: Full Text Finder (for New FTF UI) (s8985755)
    Category: fullText
    Text: Find It @ SCU Libraries
    MouseOverText: Find It @ SCU Libraries
  – Url: https://doaj.org/article/063889040e6f4076bf1a09f49bc5b6c8
    Name: EDS - DOAJ (s8985755)
    Category: fullText
    Text: View record from DOAJ
    MouseOverText: View record from DOAJ
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.063889040e6f4076bf1a09f49bc5b6c8
RelevancyScore: 942
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 942.363525390625
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Comparison of Six Antifungal Susceptibilities of 11 Candida Species Using the VITEK2 AST–YS08 Card and Broth Microdilution Method
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Hyeyoung+Lee%22">Hyeyoung Lee</searchLink><br /><searchLink fieldCode="AR" term="%22Seong+Hyouk+Choi%22">Seong Hyouk Choi</searchLink><br /><searchLink fieldCode="AR" term="%22Junsang+Oh%22">Junsang Oh</searchLink><br /><searchLink fieldCode="AR" term="%22Jehyun+Koo%22">Jehyun Koo</searchLink><br /><searchLink fieldCode="AR" term="%22Hyun+Ji+Lee%22">Hyun Ji Lee</searchLink><br /><searchLink fieldCode="AR" term="%22Sung–Il+Cho%22">Sung–Il Cho</searchLink><br /><searchLink fieldCode="AR" term="%22Jeong+Hwan+Shin%22">Jeong Hwan Shin</searchLink><br /><searchLink fieldCode="AR" term="%22Hae+Kyung+Lee%22">Hae Kyung Lee</searchLink><br /><searchLink fieldCode="AR" term="%22Soo–Young+Kim%22">Soo–Young Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Chae+Hoon+Lee%22">Chae Hoon Lee</searchLink><br /><searchLink fieldCode="AR" term="%22Young+Ree+Kim%22">Young Ree Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Yong–Hak+Sohn%22">Yong–Hak Sohn</searchLink><br /><searchLink fieldCode="AR" term="%22Woo+Jin+Kim%22">Woo Jin Kim</searchLink><br /><searchLink fieldCode="AR" term="%22Sook+Won+Ryu%22">Sook Won Ryu</searchLink><br /><searchLink fieldCode="AR" term="%22Gi–Ho+Sung%22">Gi–Ho Sung</searchLink><br /><searchLink fieldCode="AR" term="%22Jayoung+Kim%22">Jayoung Kim</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Microbiology Spectrum, Vol 10, Iss 2 (2022)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: American Society for Microbiology, 2022.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2022
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Microbiology
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Candida%22">Candida</searchLink><br /><searchLink fieldCode="DE" term="%22antifungal+susceptibility%22">antifungal susceptibility</searchLink><br /><searchLink fieldCode="DE" term="%22Vitek+2+AST–YS08%22">Vitek 2 AST–YS08</searchLink><br /><searchLink fieldCode="DE" term="%22broth+microdilution%22">broth microdilution</searchLink><br /><searchLink fieldCode="DE" term="%22epidemiological+cutoff+value%22">epidemiological cutoff value</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22QR1-502%22">QR1-502</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: ABSTRACT We used a Vitek 2 AST–YS08 (YS08) system and the broth microdilution method (BMD) adopted by the Clinical and Laboratory Standards Institute (CLSI) to compare the susceptibility of 184 isolates of 11 Candida species to fluconazole, voriconazole, micafungin, caspofungin, amphotericin B, and flucytosine. In Candida albicans, the categorical agreement (CA) was 79.2%, 91.7%, 95.8%, and 95.8% for fluconazole, voriconazole, micafungin, and caspofungin, respectively. About 12.5% and 4.2% of very major errors were detected for fluconazole and voriconazole, respectively. C. glabrata showed excellent essential agreements (EAs) (>90%) for azoles but different MIC distributions for fluconazole and caspofungin. The CA between BMD fluconazole MICs and YS08 voriconazole MICs by the method-specific clinical breakpoint (CBP) was 90% in C. glabrata. Over 80% of C. glabrata and C. krusei isolates identified as micafungin–susceptible were labeled intermediate or resistant to caspofungin in YS08. In C. parapsilosis, 5.3% of very major errors and 10.5% of minor errors were found, whereas 33.3% of minor errors were observed in C. tropicalis for fluconazole. For C. tropicalis, 13 (61.9%) non-wild type (WT) isolates of fluconazole and 7 (33.3%) non-WTs of voriconazole were classified in YS08 as WT. For C. auris, the EAs were 93.3%, 100%, 82.2%, 97.8%, and 97.8% for fluconazole, voriconazole, micafungin, caspofungin, and amphotericin B, respectively. YS08 showed comparable results to the BMD. However, considering the lower YS08 fluconazole MIC results compared with BMD in Candida species and YS08 caspofungin results in C. glabrata and C. krusei, improvements are needed. IMPORTANCE The new Vitek 2 AST–YS08 (YS08) card has been updated to reflect the recently revised Clinical and Laboratory Standards Institute (CLSI) guideline. In this study, antifungal drug susceptibility tests were performed using the YS08 card and compared with the CLSI broth microdilution (BMD) method. In conclusion, YS08 showed similar results to BMD, including with C. auris. However, about 12.5% and 4.2% of major errors were detected for fluconazole and voriconazole, respectively, in C. albicans. More than 80% of C. glabrata and C. krusei isolates identified as susceptible to micafungin were labeled moderate or resistant to caspofungin in YS08. The categorical agreement between BMD fluconazole MICs and YS08 voriconazole MICs was 90% by the method-specific CBP of voriconazole, 80% by the current epidemiological cutoff value (ECV) (0.25 μg/mL) of voriconazole, and 85% by the previous ECV (0.5 μg/mL) of voriconazole. Further improvements in YS08 for the detection of fluconazole and echinocandin resistance are thus needed.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 2165-0497
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://doaj.org/toc/2165-0497
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1128/spectrum.01253-21
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/063889040e6f4076bf1a09f49bc5b6c8" linkWindow="_blank">https://doaj.org/article/063889040e6f4076bf1a09f49bc5b6c8</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.063889040e6f4076bf1a09f49bc5b6c8
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.063889040e6f4076bf1a09f49bc5b6c8
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1128/spectrum.01253-21
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: Candida
        Type: general
      – SubjectFull: antifungal susceptibility
        Type: general
      – SubjectFull: Vitek 2 AST–YS08
        Type: general
      – SubjectFull: broth microdilution
        Type: general
      – SubjectFull: epidemiological cutoff value
        Type: general
      – SubjectFull: Microbiology
        Type: general
      – SubjectFull: QR1-502
        Type: general
    Titles:
      – TitleFull: Comparison of Six Antifungal Susceptibilities of 11 Candida Species Using the VITEK2 AST–YS08 Card and Broth Microdilution Method
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Hyeyoung Lee
      – PersonEntity:
          Name:
            NameFull: Seong Hyouk Choi
      – PersonEntity:
          Name:
            NameFull: Junsang Oh
      – PersonEntity:
          Name:
            NameFull: Jehyun Koo
      – PersonEntity:
          Name:
            NameFull: Hyun Ji Lee
      – PersonEntity:
          Name:
            NameFull: Sung–Il Cho
      – PersonEntity:
          Name:
            NameFull: Jeong Hwan Shin
      – PersonEntity:
          Name:
            NameFull: Hae Kyung Lee
      – PersonEntity:
          Name:
            NameFull: Soo–Young Kim
      – PersonEntity:
          Name:
            NameFull: Chae Hoon Lee
      – PersonEntity:
          Name:
            NameFull: Young Ree Kim
      – PersonEntity:
          Name:
            NameFull: Yong–Hak Sohn
      – PersonEntity:
          Name:
            NameFull: Woo Jin Kim
      – PersonEntity:
          Name:
            NameFull: Sook Won Ryu
      – PersonEntity:
          Name:
            NameFull: Gi–Ho Sung
      – PersonEntity:
          Name:
            NameFull: Jayoung Kim
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 04
              Type: published
              Y: 2022
          Identifiers:
            – Type: issn-print
              Value: 21650497
          Numbering:
            – Type: volume
              Value: 10
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Microbiology Spectrum
              Type: main
ResultId 1