Optimization Conditions for Ethanol Production from Sweet Sorghum Juice by Thermotolerant Yeast Saccharomyces cerevisiae: Using a Statistical Experimental Design

Bibliographic Details
Title: Optimization Conditions for Ethanol Production from Sweet Sorghum Juice by Thermotolerant Yeast Saccharomyces cerevisiae: Using a Statistical Experimental Design
Authors: Sunan Nuanpeng, Sudarat Thanonkeo, Preekamol Klanrit, Mamoru Yamada, Pornthap Thanonkeo
Source: Fermentation, Vol 9, Iss 5, p 450 (2023)
Publisher Information: MDPI AG, 2023.
Publication Year: 2023
Collection: LCC:Fermentation industries. Beverages. Alcohol
Subject Terms: ethanol production, high-temperature fermentation, sweet sorghum, thermotolerant yeast, Fermentation industries. Beverages. Alcohol, TP500-660
More Details: The optimum fermentation conditions for ethanol production from sweet sorghum juice (SSJ) by the thermotolerant yeast Saccharomyces cerevisiae DBKKUY-53 were determined using a statistical experimental design. Based on the Plackett–Burman design (PBD), yeast cell concentration, sugar concentration, and yeast extract were the significant independent fermentation factors affecting the ethanol production from SSJ at 37 °C by S. cerevisiae DBKKUY-53. These significant factors were optimized using response surface methodology (RSM) based on a central composite design (CCD). The result revealed that the optimum conditions for ethanol fermentation were 7.85 × 107 cells/mL yeast cell concentration, 247 g/L sugar concentration, and 9.99 g/L yeast extract. Verification of the ethanol production using the optimum conditions revealed that the maximum ethanol concentration of 99.75 g/L and the productivity of 2.77 g/L/h were achieved. When the ethanol production was carried out in a 2 L fermentor under optimum conditions, the ethanol concentration was 101.81 g/L and the productivity was 2.83 g/L/h. This finding suggested that the thermotolerant yeast S. cerevisiae DBKKUY-53 has excellent potential for commercial ethanol production at high temperatures.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2311-5637
Relation: https://www.mdpi.com/2311-5637/9/5/450; https://doaj.org/toc/2311-5637
DOI: 10.3390/fermentation9050450
Access URL: https://doaj.org/article/a0618b873a2d47bab54d38a0d16bf160
Accession Number: edsdoj.0618b873a2d47bab54d38a0d16bf160
Database: Directory of Open Access Journals
More Details
ISSN:23115637
DOI:10.3390/fermentation9050450
Published in:Fermentation
Language:English