Bibliographic Details
| Title: |
Differentiation of Wharton's Jelly Derived Mesenchymal Stem Cells into Insulin Producing Cells |
| Authors: |
Hossein Ranjbaran, Saeid Abediankenari, Alireza Khalilian, Zahra Rahmani, Marzei Momeninezhad Amiri, Zahra Hosseini Khah |
| Source: |
International Journal of Hematology-Oncology and Stem Cell Research, Vol 12, Iss 3 (2018) |
| Publisher Information: |
Tehran University of Medical Sciences, 2018. |
| Publication Year: |
2018 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
Mesenchymal stem cells, Wharton`s jelly, Differentiation, Insulin producing cells, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
Background: Diabetes caused by insulin production disturbance is considered as the most common metabolic disorder all over the world. Diabetes may outbreak because of low insulin secretion by Islets of Langerhans β-cells, insulin resistance or both of them. In this way, using stem cells, which have the capability to differentiate into Pancreatic β-cells, is one of novel methods in this field. MSCs are the most important candidates for cellular therapy. Materials and Methods: Insulin level was examined using ELIZA method. In order to examine the morphology of differentiated cells, they were stained by Dithizone. Insulin-producer cells are cells which turn into red as a result of staining. Specific gene involving insulin-producing cells was evaluated by Real Time-PCR method. Results: The ELISA results showed that the treated cells secreted more insulin than the control group. Moreover, we found differentiation of MSCs toward insulin-secreting cells. In order to evaluate insulin production in clusters on day 21 of differentiation, we used dithizone (DTZ) staining. PDX-1 gene was confirmed by RT- PCR analysis. Conclusion: In this study, we differentiated MSCs into insulin-producing cells in vitro. It is concluded that MSCs may be considered as an excellent candidate in β-cell therapy in diabetes patients. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2008-2207 |
| Relation: |
https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/828; https://doaj.org/toc/2008-2207 |
| Access URL: |
https://doaj.org/article/05aa5154af65465eb196398ee80b9adb |
| Accession Number: |
edsdoj.05aa5154af65465eb196398ee80b9adb |
| Database: |
Directory of Open Access Journals |
