Bibliographic Details
| Title: |
Preparation of mitochondria to measure superoxide flashes in angiosperm flowers |
| Authors: |
Chulan Zhang, Fengshuo Sun, Biao Xiong, Zhixiang Zhang |
| Source: |
PeerJ, Vol 7, p e6708 (2019) |
| Publisher Information: |
PeerJ Inc., 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Medicine
LCC:Biology (General) |
| Subject Terms: |
Isolation of mitochondria, Mitochondrial membrane potential (ΔΨm), ROS, Superoxide flashes, Medicine, Biology (General), QH301-705.5 |
| More Details: |
Background Mitochondria are the center of energy metabolism and the production of reactive oxygen species (ROS). ROS production results in a burst of “superoxide flashes”, which is always accompanied by depolarization of mitochondrial membrane potential. Superoxide flashes have only been studied in the model plant Arabidopsis thaliana using a complex method to isolate mitochondria. In this study, we present an efficient, easier method to isolate functional mitochondria from floral tissues to measure superoxide flashes. Method We used 0.5 g samples to isolate mitochondria within |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2167-8359 |
| Relation: |
https://peerj.com/articles/6708.pdf; https://peerj.com/articles/6708/; https://doaj.org/toc/2167-8359 |
| DOI: |
10.7717/peerj.6708 |
| Access URL: |
https://doaj.org/article/055efb90c52d4ee98fbc705e46dd3c2b |
| Accession Number: |
edsdoj.055efb90c52d4ee98fbc705e46dd3c2b |
| Database: |
Directory of Open Access Journals |
