Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor
| Title: | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
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| Authors: | Verena Siewers, Muriel Viaud, Daniel Jimenez-Teja, Isidro G. Collado, Christian Schulze Gronover, Jean-Marc Pradier, Bettina Tudzynsk, Paul Tudzynski |
| Source: | Molecular Plant-Microbe Interactions, Vol 18, Iss 6, Pp 602-612 (2005) |
| Publisher Information: | The American Phytopathological Society, 2005. |
| Publication Year: | 2005 |
| Collection: | LCC:Microbiology LCC:Botany |
| Subject Terms: | secondary metabolism, signaling, Microbiology, QR1-502, Botany, QK1-989 |
| More Details: | The micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization of host tissue is accompanied by rapid killing of plant cells ahead of the growing hyphen, probably caused by secretion of nonspecific phytotoxins, e.g., the sesquiterpene botrydial. Although all pathogenic strains tested so far had been shown to secrete botrydial and although the toxin causes comparable necrotic lesions as infection by the fungus, the role of botrydial in the infection process has not been elucidated so far. Here, we describe the functional characterization of bcbot1, encoding a P450 monooxygenase and provide evidence that it is involved in the botrydial pathway, i.e., it represents the first botrydial biosynthetic gene identified. We show that bcbot1 is expressed in planta and that expression in vitro and in planta is controlled by an α-subunit of a heterotrimeric GTP-binding protein, BCG1. Deletion of bcbot1 in three standard strains of B. cinerea shows that the effect on virulence (on several host plants) is strain-dependent; only deletion in one of the strains (T4) led to reduced virulence. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-18-0602 |
| Access URL: | https://doaj.org/article/da050c517b1c4271b9dc0bce3724faeb |
| Accession Number: | edsdoj.050c517b1c4271b9dc0bce3724faeb |
| Database: | Directory of Open Access Journals |
| ISSN: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-18-0602 |
| Published in: | Molecular Plant-Microbe Interactions |
| Language: | English |