Bibliographic Details
| Title: |
Repeat HIV testing of individuals with discrepant HIV self-test results in Central Uganda |
| Authors: |
Rose Kisa, Joseph K. B. Matovu, Esther Buregyeya, William Musoke, Caroline J. Vrana-Diaz, Jeffrey E. Korte, Rhoda K. Wanyenze |
| Source: |
AIDS Research and Therapy, Vol 16, Iss 1, Pp 1-7 (2019) |
| Publisher Information: |
BMC, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Immunologic diseases. Allergy |
| Subject Terms: |
HIV self-testing, Weak band, Confirmatory HIV testing, Uganda, Immunologic diseases. Allergy, RC581-607 |
| More Details: |
Abstract Background According to the user instructions from the manufacturer of OraQuick HIV self-test (HIVST) kits, individuals whose kits show one red band should be considered to be HIV-negative, no matter how weak the band is. However, recent reports show potential for a second false weak band after storage, thereby creating confusion in the interpretation of results. In this study, we re-tested individuals whose results were initially non-reactive but changed to weak reactive results to determine their true HIV status. Methods This study was nested within a large, cluster-randomized HIVST trial implemented among pregnant women attending antenatal care and their male partners in central Uganda between July 2016 and February 2017. Ninety-five initially HIV-negative respondents were enrolled into this study, including 52 whose kits developed a second weak band while in storage and 43 whose kits were interpreted as HIV-positive by interviewers at the next follow-up interview. Respondents were invited to return for repeat HIVST which was performed under the observation of a trained nurse counsellor. After HIVST, respondents underwent blood-based rapid HIV testing as per the national HIV testing algorithm (Determine (Abbot Laboratories), STAT-PAK (Chembio Diagnostic Systems Inc.) and Unigold (Trinity Biotech plc.) and dry blood spots were obtained for DNA/PCR testing. DNA/PCR was considered as the gold-standard HIV testing method. Results After repeat HIVST, 90 (94.7%) tested HIV-negative; 2 (2.1%) tested HIV-positive; and 3 (3.2%) had missing HIV test results. When respondents were subjected to blood-based rapid HIV testing, 97.9% (93/95) tested HIV-negative while 2.1% (2/95) tested HIV-positive. Finally, when the respondents were subjected to DNA/PCR, 99% (94/95) tested HIV-negative while 1.1% (1/95) tested HIV-positive. Conclusions Nearly all initially HIV-negative individuals whose HIVST kits developed a second weak band while in storage or were interpreted as HIV-positive by interviewers were found to be HIV-negative after confirmatory DNA/PCR HIV testing. These findings suggest a need for HIV-negative individuals whose HIVST results change to false positive while under storage or under other sub-optimal conditions to be provided with an option for repeat testing to determine their true HIV status. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1742-6405 |
| Relation: |
http://link.springer.com/article/10.1186/s12981-019-0243-1; https://doaj.org/toc/1742-6405 |
| DOI: |
10.1186/s12981-019-0243-1 |
| Access URL: |
https://doaj.org/article/cec04f5e4f0d4b3f84b7cba3ccb255e7 |
| Accession Number: |
edsdoj.04f5e4f0d4b3f84b7cba3ccb255e7 |
| Database: |
Directory of Open Access Journals |
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