Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and Northeastern Colombia
| Title: | Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and Northeastern Colombia |
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| Authors: | Carmen Elisa Posso, Ranulfo González, Heiber Cárdenas, Gerardo Gallego, Myriam Cristina Duque, Marco Fidel Suarez |
| Source: | Memorias do Instituto Oswaldo Cruz, Vol 98, Iss 4, Pp 469-476 (2003) |
| Publisher Information: | Fundação Oswaldo Cruz (FIOCRUZ), 2003. |
| Publication Year: | 2003 |
| Collection: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| Subject Terms: | Anopheles nuneztovari, genetic diversity, malaria vectors, Nyssorrhynchus, ramdon amplified polymorphic DNA, polymerase chain reaction, Colombia, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| More Details: | Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (F ST: 0.035; Nm: 6.8) but lower between these two and the northeastern population (F ST: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (phiST 0.1131, P < 0.001). The variation among individuals within populations (phiST 0.8869, P < 0.001)was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 0074-0276 1678-8060 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000400007; https://doaj.org/toc/0074-0276; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/S0074-02762003000400007 |
| Access URL: | https://doaj.org/article/03e25b9ad8b14996a54ba8260e1932fa |
| Accession Number: | edsdoj.03e25b9ad8b14996a54ba8260e1932fa |
| Database: | Directory of Open Access Journals |
| ISSN: | 00740276 16788060 |
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| DOI: | 10.1590/S0074-02762003000400007 |
| Published in: | Memorias do Instituto Oswaldo Cruz |
| Language: | English |