Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures.

Bibliographic Details
Title:	Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures.
Authors:	Mingjie Tang, Mingkun Zhang, Shihan Yan, Liangping Xia, Zhongbo Yang, Chunlei Du, Hong-Liang Cui, Dongshan Wei
Source:	PLoS ONE, Vol 13, Iss 1, p e0191515 (2018)
Publisher Information:	Public Library of Science (PLoS), 2018.
Publication Year:	2018
Collection:	LCC:Medicine LCC:Science
Subject Terms:	Medicine, Science
More Details:	DNA oligonucleotides with a 5-base mutation at the 3'-terminus were investigated by terahertz (THz) spectroscopy in a marker-free manner. The four single-stranded oligonucleotides with 17nt have been detected with specificity on a microfluidic chip, and corroborated by spectral measurements with split-ring resonators. The number of hydrogen bonds formed between the oligonucleotide and its surrounding water molecules, deemed a key contribution to the THz absorption of biological solutions, was explored by molecular dynamics simulations to explain the experimental findings. Our work underlies the feasibility of THz spectroscopy combined with microstructures for marker-free detection of DNA, which may form the basis of a prospective diagnostic tool for studying genic mutation.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	1932-6203
Relation:	http://europepmc.org/articles/PMC5783420?pdf=render; https://doaj.org/toc/1932-6203
DOI:	10.1371/journal.pone.0191515
Access URL:	https://doaj.org/article/c03e04f755284982b76101daf0cd2b6e
Accession Number:	edsdoj.03e04f755284982b76101daf0cd2b6e
Database:	Directory of Open Access Journals
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More Details
ISSN:	19326203
DOI:	10.1371/journal.pone.0191515
Published in:	PLoS ONE
Language:	English