Bibliographic Details
| Title: |
Synthesis of Headful Packaging Phages Through Yeast Transformation-Associated Recombination |
| Authors: |
Cheng Lu, Lan He, Yangyijun Guo, Tingting Wang, Yanrui Ye, Zhanglin Lin |
| Source: |
Viruses, Vol 17, Iss 1, p 45 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
headful packaging, synthetic phage, yeast transformation-associated recombination, Pseudomonas aeruginosa phage, Microbiology, QR1-502 |
| More Details: |
De novo synthesis of phage genomes enables flexible genome modification and simplification. This study explores the synthetic genome assembly of Pseudomonas phage vB_PaeS_SCUT-S4 (S4), a 42,932 bp headful packaging phage, which encapsidates a terminally redundant, double-stranded DNA genome exceeding unit length. We demonstrate that using the yeast TAR approach, the S4 genome can be assembled and rebooted from a unit-length genome plus a minimal 60 bp terminal redundant sequence. Furthermore, we show that S4 can be synthesized from arbitrary starting nucleotides and modified with a red fluorescent protein as a reporter. Additionally, we successfully designed and assembled synthetic S4 phages with reduced genomes, knocking out up to 10 of the 24 hypothetical genes simultaneously, with a combined length of 2883 bp, representing 6.7% of the unit-length genome. This work highlights the potential for engineering simplified, customizable headful packaging phage genomes, providing a foundation for future studies of these phages for potential clinical applications. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1999-4915 |
| Relation: |
https://www.mdpi.com/1999-4915/17/1/45; https://doaj.org/toc/1999-4915 |
| DOI: |
10.3390/v17010045 |
| Access URL: |
https://doaj.org/article/03b8b7f9630f4a7bba7c616d3bd12676 |
| Accession Number: |
edsdoj.03b8b7f9630f4a7bba7c616d3bd12676 |
| Database: |
Directory of Open Access Journals |
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