Bibliographic Details
| Title: |
Proteomic Identification of an Upregulated Isoform of Annexin A3 in the Spinal Cords of Rats in a Neuropathic Pain Model |
| Authors: |
Wangyuan Zou, Wei Xu, Zongbin Song, Tao Zhong, Yingqi Weng, Changsheng Huang, Maoyu Li, Chuanlei Zhang, Xianquan Zhan, Qulian Guo |
| Source: |
Frontiers in Neuroscience, Vol 11 (2017) |
| Publisher Information: |
Frontiers Media S.A., 2017. |
| Publication Year: |
2017 |
| Collection: |
LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| Subject Terms: |
neuropathic pain, spinal cord, proteomics, ANXA3, microglia, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| More Details: |
Neuropathic pain (NP) is induced by nerve damage or a disturbance in the peripheral or central nervous systems. Nerve damage causes the activation of sensitizing mechanisms in the peripheral and central nervous systems, which induces transcriptional and post-transcriptional alterations in sensory nerves. However, the underlying mechanisms of NP remain elusive. In the study, Two-dimensional gel electrophoresis (2DGE)-based comparative proteomics identified 38 differential gel spots, and 15 differentially expressed proteins (DEPs) between the sham and the chronic constriction injury (CCI)-induced neuropathic pain rats. Of them, Annexin A3 (ANXA3) was significantly increased after CCI with Western blot analysis and immunofluorescence imaging. A lentivirus delivering ANXA3 shRNA (LV-shANXA3) was administered intrathecally to determine the analgesic effects of ANXA3 on allodynia and hyperalgesia in a CCI-induced neuropathic pain model in rats. Further study showed that LV-shANXA3 reversed the upregulation of ANXA3, alleviated CCI-induced mechanical allodynia and thermal hyperalgesia. The study indicated that ANXA3 may play an important role in neuropathic pain. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1662-453X |
| Relation: |
http://journal.frontiersin.org/article/10.3389/fnins.2017.00484/full; https://doaj.org/toc/1662-453X |
| DOI: |
10.3389/fnins.2017.00484 |
| Access URL: |
https://doaj.org/article/0386d40deebd454d8fd4ef16afcd7244 |
| Accession Number: |
edsdoj.0386d40deebd454d8fd4ef16afcd7244 |
| Database: |
Directory of Open Access Journals |
