Plasma and tissue angiotensin‐converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease
| Title: | Plasma and tissue angiotensin‐converting enzyme 2 activity and plasma equilibrium concentrations of angiotensin peptides in dogs with heart disease |
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| Authors: | Éva Larouche‐Lebel, Kerry A. Loughran, Mark A. Oyama, Phil F. Solter, Danielle S. Laughlin, Melissa D. Sánchez, Charles‐Antoine Assenmacher, Philip R. Fox, Ryan C. Fries |
| Source: | Journal of Veterinary Internal Medicine, Vol 33, Iss 4, Pp 1571-1584 (2019) |
| Publisher Information: | Wiley, 2019. |
| Publication Year: | 2019 |
| Collection: | LCC:Veterinary medicine |
| Subject Terms: | angiotensin 1‐7, angiotensin II, degenerative mitral valve disease, renin‐angiotensin‐aldosterone system, Veterinary medicine, SF600-1100 |
| More Details: | Abstract Background Angiotensin‐converting enzyme 2 (ACE2) is a homologue of angiotensin‐converting enzyme (ACE) and produces angiotensin peptides (APs), such as angiotensin 1‐9 and 1‐7 that are vasodilatory and natriuretic, and act to counterbalance angiotensin II. Hypothesis Evidence of ACE2 can be found in tissues and plasma of dogs. Equilibrium concentrations of renin angiotensin aldosterone system (RAAS) APs differ in dogs with heart disease compared to healthy dogs and recombinant human ACE2 (rhACE2) alters relative concentrations of APs. Animals Forty‐nine dogs with and 34 dogs without heart disease. Methods Immunohistochemistry and assays for tissue and plasma ACE2 activity and equilibrium concentrations of plasma RAAS APs were performed. Results Immunolabeling for ACE2 was present in kidney and myocardial tissue. Median plasma ACE2 activity was significantly increased in dogs with congestive heart failure (CHF; 6.9 mU/mg; interquartile range [IQR], 5.1‐12.1) as compared to control (2.2 mU/mg; IQR, 1.8‐3.0; P = .0003). Plasma equilibrium analysis of RAAS APs identified significant increases in the median concentrations of beneficial APs, such as angiotensin 1‐7, in dogs with CHF (486.7 pg/mL; IQR, 214.2‐1168) as compared to those with preclinical disease (41.0 pg/mL; IQR, 27.4‐45.1; P |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1939-1676 0891-6640 |
| Relation: | https://doaj.org/toc/0891-6640; https://doaj.org/toc/1939-1676 |
| DOI: | 10.1111/jvim.15548 |
| Access URL: | https://doaj.org/article/02b3d8c4ae68407f976d0e8cf1b5ad1f |
| Accession Number: | edsdoj.02b3d8c4ae68407f976d0e8cf1b5ad1f |
| Database: | Directory of Open Access Journals |
| ISSN: | 19391676 08916640 |
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| DOI: | 10.1111/jvim.15548 |
| Published in: | Journal of Veterinary Internal Medicine |
| Language: | English |